microbial 2

Question 1

main source of nitrogen for prokaryotic cells

Answer 1

-atmospheric nitrogen
-N2 Ammonia NH3
- nitrate NO3, NO2 of organic compounds

Question 2

potassium roles in prokaryotes

Answer 2

-osmoregulation
- enzyme activity
- nerve function

Question 3

magnesium roles in prokaryotes

Answer 3

-structural role
- enzyme activity
- cell division

Question 4

macronutrients required by microorg

Answer 4

in large quantities- CHONPS
carbon, hydrogen, oxygen, nitrogen, phosphorus, sulfur

Question 5

macronutrients needed in lesser amounts

Answer 5

Ga +2, Fe +3, Mg +2, K

Question 6

microelements (trace elements)

Answer 6

small amounts
Fe, Zn, copper, maganese, Mo

Question 7

growth factors

Answer 7

small molecules which are not used for energy source but specifically required for growth
- Amino acids
- Purines
- Pyrimindes
- vitimins

Question 8

liquid media is used when

Answer 8

a large number of microbes or microbial products is needed
- culturing microorg
- testing metabolic activity

Question 9

solid medium is used when

Answer 9

• isolation of individual species
• bacterial counting
• antibiotic resistance testing

Question 10

agar once melted does not harden until

Answer 10

44C
and melt at 90C

Question 10

agar is

Answer 10

solidifying agent - room temp, stable surface for microbial growth

Question 11

Necessities for growth of all prokaryotes in most media

Answer 11

-water
-source of energy
- nitrogen
- inorganic ions
- trace elements

Question 12

4 main types of culture media

Answer 12

• defined minimal media
• complex medium
• selective medium
• enrichment medium