Micro Plates Flashcards
Supportive Media
Supports growth of most nonfastidious bacteria
Enrichment Media
contains growth factors such as blood, vitamins and yeast extract
Selective Media
Contains additives to inhibit growth of certain bacteria
What additives are in selective media?
dyes, bile salts, alcohols, antibiotics, acids
Differential Media
formulates to produce distinct colonial appearances based on chemical reactions such as lactose, fermentation and H2S production.
Sheep Blood Agar
Enriched differential media based on hemolysis. Used mostly on Strep annd Staph.
Chocolate Agar
Enrichment Media used for isolation of Neisseria and Haemophilus influenzae
CNA (Columbia Colistin-Nalidixic acid agar)
Selective media with acid to suppress Gram neg bacteria. Isolates gram pos cocci
Phenylethyl alcohol agar (PEA)
Selective media with alcohol to inhibit Proteus. isolation of gram pos cocci and anaerobic gram neg rods
Selective Strep Agaar (SSA)
For isolation of group A strep; contains antibioltics to suppress throat flora
Eosin methylene blue (EMB)
selective and differential; inhibit Gram pos: isolate gram neg rods by fermentation
MacConkey (MAC)
selective and differential; isolation fof gram neg rods with bile salts and crystal violet. Fermenters are pink.
Sorbitol MacConkey (SMAC)
Isolation of E.coli 0157:H7; cannot ferment: colorless colonies
Hektoen Enteric Agar (HE)
Isolation of Salmonella and Shigella in stool
Reactions of HE agar
Bile salts, bromothymol blue and acid fuchsin inhibit GI flora. Non pathogens are orange/pink. non lactose fermenters blue/green. H2S black precipitate.
Xylose lysine deoxycholate (XLD)
isolation of Salmonella and Shigella in stool
E. Coli on XLD
Yellow
Proteus on XLD
Yellow with black centers
Shigella on XLD
Colorless of Red colonies
Salmonella on XLD
Red colonies with black centers
Salmonella-Shigella agar (SS)
Isolation of Salmonella and Shigella in stool
brilliant green and bile salts inhibit other enterics.
Salmonella on SS
black center from H2S
Shigella on SS
colorless colonies
Gram Negative Broth (GN)
isolation of Salmonella and Shigella from stools and rectal swabs
Deoxycholate citrate agar
isolation of salmonella and shigella
Campy Broth
isolation of Campylobacter from stool in overnight inc 4C
Campy BAP
Isolation of Campylobacter from stool
incubate in gas chamber CO2 at 42C
Salmonella on HE
Green with black centers H2S positive
Shigella on HE
Green colonies
Sabouraud Dextrose Brain Heart Infusion Agar (SabHI)
Primary recovery of saprophytic and dimorphic fungi
Sabouraud Agar (SAB)
selective medium that is formulated to allow growth of fungi(dermatophytes) and inhibit the growth of bacteria.
Brain heart Infustion agar (BHI)
a rich medium for bacteria, yeast, and pathogenic fungi; primary recovery of fungi and Actinomycetales
Thioglycollate (THIO)
Grows aerobic, anaerobic and microaerophilic organisms
Bile esculin azide w/ vanco
selective media for isolation of vanco resistant enterococci
Spectra MRSA
isolation of oxacillin resistant S. aureus
BCSA
Burkholderia cepacia Selective Agar for isolation of Burkholderia cepacia in CF patients
Lim broth (LIM)
enrichment media enhances growth of group B strep
Thayer Martin (TM)
selective agar for growth of pathogenic neisseria
Bacteroides bile esculin (BBE)
isolation of B. fragilis
ANA
isolation of anaerobic organisms
supplemented with Vit k1, hemin and cystine
LKV
selective agar for isolation of anaerobic gram negative species specifically Bacteroides.
Buffered charcoal yeast extract (BCYE)
isolation of legionella
Mycobiotic Agar
isolation of pathogenic fungi from sources with bacteria
Inhibitory Mold Agar
isolation of pathogenic fungi from sources with bacteria
Bile esculin agar
Identify enterococcus and group D strep
Esculin is hydrolysis with iron and turns black
