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Microbiology > Micro Plates > Flashcards

Micro Plates Flashcards

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Biology 101 flashcards
1
Q

Supportive Media

A

Supports growth of most nonfastidious bacteria

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2
Q

Enrichment Media

A

contains growth factors such as blood, vitamins and yeast extract

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3
Q

Selective Media

A

Contains additives to inhibit growth of certain bacteria

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4
Q

What additives are in selective media?

A

dyes, bile salts, alcohols, antibiotics, acids

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5
Q

Differential Media

A

formulates to produce distinct colonial appearances based on chemical reactions such as lactose, fermentation and H2S production.

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6
Q

Sheep Blood Agar

A

Enriched differential media based on hemolysis. Used mostly on Strep annd Staph.

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7
Q

Chocolate Agar

A

Enrichment Media used for isolation of Neisseria and Haemophilus influenzae

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8
Q

CNA (Columbia Colistin-Nalidixic acid agar)

A

Selective media with acid to suppress Gram neg bacteria. Isolates gram pos cocci

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9
Q

Phenylethyl alcohol agar (PEA)

A

Selective media with alcohol to inhibit Proteus. isolation of gram pos cocci and anaerobic gram neg rods

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10
Q

Selective Strep Agaar (SSA)

A

For isolation of group A strep; contains antibioltics to suppress throat flora

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11
Q

Eosin methylene blue (EMB)

A

selective and differential; inhibit Gram pos: isolate gram neg rods by fermentation

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12
Q

MacConkey (MAC)

A

selective and differential; isolation fof gram neg rods with bile salts and crystal violet. Fermenters are pink.

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13
Q

Sorbitol MacConkey (SMAC)

A

Isolation of E.coli 0157:H7; cannot ferment: colorless colonies

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14
Q

Hektoen Enteric Agar (HE)

A

Isolation of Salmonella and Shigella in stool

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15
Q

Reactions of HE agar

A

Bile salts, bromothymol blue and acid fuchsin inhibit GI flora. Non pathogens are orange/pink. non lactose fermenters blue/green. H2S black precipitate.

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16
Q

Xylose lysine deoxycholate (XLD)

A

isolation of Salmonella and Shigella in stool

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17
Q

E. Coli on XLD

A

Yellow

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18
Q

Proteus on XLD

A

Yellow with black centers

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19
Q

Shigella on XLD

A

Colorless of Red colonies

20
Q

Salmonella on XLD

A

Red colonies with black centers

21
Q

Salmonella-Shigella agar (SS)

A

Isolation of Salmonella and Shigella in stool

brilliant green and bile salts inhibit other enterics.

22
Q

Salmonella on SS

A

black center from H2S

23
Q

Shigella on SS

A

colorless colonies

24
Q

Gram Negative Broth (GN)

A

isolation of Salmonella and Shigella from stools and rectal swabs

25
Q

Deoxycholate citrate agar

A

isolation of salmonella and shigella

26
Q

Campy Broth

A

isolation of Campylobacter from stool in overnight inc 4C

27
Q

Campy BAP

A

Isolation of Campylobacter from stool

incubate in gas chamber CO2 at 42C

28
Q

Salmonella on HE

A

Green with black centers H2S positive

29
Q

Shigella on HE

A

Green colonies

30
Q

Sabouraud Dextrose Brain Heart Infusion Agar (SabHI)

A

Primary recovery of saprophytic and dimorphic fungi

31
Q

Sabouraud Agar (SAB)

A

selective medium that is formulated to allow growth of fungi(dermatophytes) and inhibit the growth of bacteria.

32
Q

Brain heart Infustion agar (BHI)

A

a rich medium for bacteria, yeast, and pathogenic fungi; primary recovery of fungi and Actinomycetales

33
Q

Thioglycollate (THIO)

A

Grows aerobic, anaerobic and microaerophilic organisms

34
Q

Bile esculin azide w/ vanco

A

selective media for isolation of vanco resistant enterococci

35
Q

Spectra MRSA

A

isolation of oxacillin resistant S. aureus

36
Q

BCSA

A

Burkholderia cepacia Selective Agar for isolation of Burkholderia cepacia in CF patients

37
Q

Lim broth (LIM)

A

enrichment media enhances growth of group B strep

38
Q

Thayer Martin (TM)

A

selective agar for growth of pathogenic neisseria

39
Q

Bacteroides bile esculin (BBE)

A

isolation of B. fragilis

40
Q

ANA

A

isolation of anaerobic organisms

supplemented with Vit k1, hemin and cystine

41
Q

LKV

A

selective agar for isolation of anaerobic gram negative species specifically Bacteroides.

42
Q

Buffered charcoal yeast extract (BCYE)

A

isolation of legionella

43
Q

Mycobiotic Agar

A

isolation of pathogenic fungi from sources with bacteria

44
Q

Inhibitory Mold Agar

A

isolation of pathogenic fungi from sources with bacteria

44
Q

Bile esculin agar

A

Identify enterococcus and group D strep

Esculin is hydrolysis with iron and turns black

Microbiology (6 decks)

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