Micro lab quiz 1 Flashcards
15 (T/F) 15 (multiple choice) 6 short answer
Compound microscope uses_____sets of lenses
2
first compound microscope was made ______ and it magnified______
1600…..3x9 times
first man made magnifying lens dates back to…
1267
scan lense magnification
lens:
mag of ocular:
total mag:
lens: 4x
mag of ocular: 10x
total mag: 40x
high power lens
made in:
magnifies:
made in: 1830s
magnifies: 400x
the ocular lens magnifies____
x10
compound microscope uses _________ sets of lenses
2
Oil immersion lens
made in:
magnifies:
made in: 1860s
magnifies: 1000x
allowing us to study bacterial morphology
types of objective lenses
(closest to the specimen)
scan
low power
high (dry) power
oil immersions
low power lense magnification
lens:
mag of ocular:
total mag:
lens: 10x
mag of ocular: 10x
total mag: 100x
high (dry) power lense magnification
lens:
mag of ocular:
total mag:
lens: 40x
mag of ocular: 10x
total mag: 400x
oil immersions lense magnification
lens:
mag of ocular:
total mag:
lens: 100x
mag of ocular: 10x
total mag: 1000x
working distance definition
the distance between the objective lens and the specimen being observed is called working distance
As magnification increases working distance decreases
true/false
check this
true
As magnification increases working distance increases
true/false
true
What is field of vision?
the area or portion of the specimen that is seen through the microscope (virtual image)
as magnification increases field of vision decreases
true/false
true
adjust diaphragm everytime you _________ magnification
increase
a specimen is placed on a…
slide
function of condenser lens?
- to bend light rays so they enter the objective lenses
- if condenser lens is not used , the light rays bend at such great angles they would not enter the objective lens
- not used in oil immersion lense
Where is the IRIS DIAPHRAGM located ?
-betweeen the condenser and the light sources
- move lever to right to decrease light
- move to left to increase lighting
when do you adjust the iris diaphragm to increase amount of light ?
- when changing objective lense for higher magnification
(t/f) the size of the opening of the objective lenses decreases as magnification increases.
true
- that’s why we increase the light using iris diaphragm at for higher magnification lenses
why do we do GRAM STAINS?
- it separates bacteria into 2 categories based on cell wall structure
gram negative = red
gram positive= purple
