Micro LAB Flashcards

1
Q

Taxo N

A

Oxidase

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2
Q

Taxo P

A

optochin

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3
Q

Taxo A

A

Bacitracin

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4
Q

S. pneumoniae medium + for swelling

A

Neufield Quellung

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5
Q

Etiologic agent of whooping cough

A

Bordatella pertussis

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6
Q

Culture media with Neutral Red

A

MAC
SSA

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7
Q

Culture media with Phenol Red

A

MSA
XLD
CTA
Urease
TSI/KIA

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8
Q

Culture media with Bromthymol Blue

A

Citrate
HEA
OF
TCBS

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9
Q

Acid (Orange-yellow) bacteria

A

V. cholerae

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10
Q

Alkaline (blue-green) bacteria

A

V. parahemolytics

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11
Q

Rapid Lactose Fermenters

A

Escherichia coli
Klebsiella - mucoid
Enterobacter

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12
Q

Late Lactose Fermenters

A

Citrobacter
Serratia
Salmonella arizonae
Shigella sonnei
Hafria
Yersinia

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13
Q

INDOLE (+)

A

MPEKPECP

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14
Q

METHYL RED (-) VP (+)

A

KEESH

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15
Q

CITRATE (+)

A

PCHEEKSS

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16
Q

RAPID UREASE (+)

A

PPM

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17
Q

H2S (+)

A

Black

SPACE

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18
Q

Mcfarland standard turbidity

A

0.5

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19
Q

Method used for Mueller Hinton agar

A

Kirby-Bauer method (disk diffusion)

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20
Q

Gram (-) cocci

A

NVM

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21
Q

color of beta hemolytic pattern

A

Yellow

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22
Q

color of alpha hemolytic pattern

A

colorless/green

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23
Q

gamma hemolytic pattern

A

no pattern seen in gamma

24
Q

bacteria with double hemolytic pattern

A

C. perfringens

25
Q

Culture media for Brucella

A

Castaneda agar

26
Q

For beta hemolytic, which test is used for susceptibility

A

Bacitracin

27
Q

Bacitracin (S)

A

S. pyogenes

28
Q

Bacitracin (R)

A

Perform CAMP/Hippurate test

29
Q

CAMP/Hippurate test (+)
Beta-hemolytic

A

S. agalactiae

30
Q

CAMP/Hippurate test (-)
beta hemolytic

A

Bile Esculin Hydrolysis test
Group D (+)

31
Q

which test to perform after Group D (+)

A

perform 6.5% NaCl/PYR

32
Q

6.5% NaCl/PYR (+)

A

Enterococcus

33
Q

6.5% NaCl/PYR (-)

A

S. bovis

34
Q

Arrowhead in CAMP
beta hemolytic

A

S. aureus

35
Q

Double arrowhead in reverse CAMP
beta hemolytic

A

C. perfringens
A. haemolyticum

36
Q

box head in reverse CAMP
beta hemolytic

A

L. monocytogenes

37
Q

which test to perform on Alpha-hemolytic pattern bacteria

A

Optochin

38
Q

Optochin (S)

A

S. pneumoniae

39
Q

Susceptibility test for S. pneumoniae alpha hemolytic

A

MH

40
Q

Optochin (R)

A

Perform Bile Esculin hydrolysis (S. viridans)

41
Q

What causes the D zone?

A

erm gene

42
Q

Biological indicator of autoclave

A

B. stearothermophilus

43
Q

Biological indicator of Ionizing radiation

A

B. pumilis

44
Q

Biological indicator of Dry Heat

A

B. subtilis var. niger

45
Q

Biological indicator of Ethylene Glycol

A

B. subtilis var. globitii

46
Q

Test for Helicobacter pylori

A

Urea breath test

47
Q

Double stranded DNA viruses

A

Pa Ad Po He He

48
Q

RNA viruses

A

Reoviridae

49
Q

What detects southern blot

A

DNA

50
Q

What detects northern blot

A

RNA

51
Q

what detects western blot

A

proteins

52
Q

example of selective media

A

MAC (only gram. neg)

53
Q

which media should be autoclaved first?

A

MAC
TSA

54
Q

which media should be dispensed in bottle first?

A

THIO
BHI
Lauryl broth

55
Q

used to differentiate Neisseria

A

Carb fermentation

56
Q

AFB how many fields

A

300

57
Q

bac. no. of E. coli in stool

A

0157H7
Enteropathogenic E. coli