Micro LAB Flashcards
Taxo N
Oxidase
Taxo P
optochin
Taxo A
Bacitracin
S. pneumoniae medium + for swelling
Neufield Quellung
Etiologic agent of whooping cough
Bordatella pertussis
Culture media with Neutral Red
MAC
SSA
Culture media with Phenol Red
MSA
XLD
CTA
Urease
TSI/KIA
Culture media with Bromthymol Blue
Citrate
HEA
OF
TCBS
Acid (Orange-yellow) bacteria
V. cholerae
Alkaline (blue-green) bacteria
V. parahemolytics
Rapid Lactose Fermenters
Escherichia coli
Klebsiella - mucoid
Enterobacter
Late Lactose Fermenters
Citrobacter
Serratia
Salmonella arizonae
Shigella sonnei
Hafria
Yersinia
INDOLE (+)
MPEKPECP
METHYL RED (-) VP (+)
KEESH
CITRATE (+)
PCHEEKSS
RAPID UREASE (+)
PPM
H2S (+)
Black
SPACE
Mcfarland standard turbidity
0.5
Method used for Mueller Hinton agar
Kirby-Bauer method (disk diffusion)
Gram (-) cocci
NVM
color of beta hemolytic pattern
Yellow
color of alpha hemolytic pattern
colorless/green
gamma hemolytic pattern
no pattern seen in gamma
bacteria with double hemolytic pattern
C. perfringens
Culture media for Brucella
Castaneda agar
For beta hemolytic, which test is used for susceptibility
Bacitracin
Bacitracin (S)
S. pyogenes
Bacitracin (R)
Perform CAMP/Hippurate test
CAMP/Hippurate test (+)
Beta-hemolytic
S. agalactiae
CAMP/Hippurate test (-)
beta hemolytic
Bile Esculin Hydrolysis test
Group D (+)
which test to perform after Group D (+)
perform 6.5% NaCl/PYR
6.5% NaCl/PYR (+)
Enterococcus
6.5% NaCl/PYR (-)
S. bovis
Arrowhead in CAMP
beta hemolytic
S. aureus
Double arrowhead in reverse CAMP
beta hemolytic
C. perfringens
A. haemolyticum
box head in reverse CAMP
beta hemolytic
L. monocytogenes
which test to perform on Alpha-hemolytic pattern bacteria
Optochin
Optochin (S)
S. pneumoniae
Susceptibility test for S. pneumoniae alpha hemolytic
MH
Optochin (R)
Perform Bile Esculin hydrolysis (S. viridans)
What causes the D zone?
erm gene
Biological indicator of autoclave
B. stearothermophilus
Biological indicator of Ionizing radiation
B. pumilis
Biological indicator of Dry Heat
B. subtilis var. niger
Biological indicator of Ethylene Glycol
B. subtilis var. globitii
Test for Helicobacter pylori
Urea breath test
Double stranded DNA viruses
Pa Ad Po He He
RNA viruses
Reoviridae
What detects southern blot
DNA
What detects northern blot
RNA
what detects western blot
proteins
example of selective media
MAC (only gram. neg)
which media should be autoclaved first?
MAC
TSA
which media should be dispensed in bottle first?
THIO
BHI
Lauryl broth
used to differentiate Neisseria
Carb fermentation
AFB how many fields
300
bac. no. of E. coli in stool
0157H7
Enteropathogenic E. coli