MICRO chp 7 Flashcards

1
Q

What is sterilization?

A

Destruction and/or removal of all microbes and their spores/cysts.

Prions may still survive.

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2
Q

Define vegetative microbe.

A

A microbe in a functional state, capable of growth and reproduction.

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3
Q

What is a spore/cyst?

A

A microbe in a hibernating state, capable of surviving extreme conditions.

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4
Q

What does it mean for a spore to germinate?

A

To turn back into vegetative microbes when certain environmental conditions occur.

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5
Q

What is sepsis?

A

Microbial contamination.

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6
Q

What is commercial sterilization?

A

Conditions sufficient to destroy endospores of Clostridium botulinum.

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7
Q

How is commercial sterilization different from regular sterilization?

A

More limited than regular sterilization.

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8
Q

What is sanitization?

A

Removal or destruction of vegetative microbes to low enough levels for public safety.

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9
Q

What is disinfection?

A

Destruction of all vegetative microbes.

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10
Q

What is antisepsis?

A

Disinfection of living tissue.

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11
Q

Define degermation.

A

Mechanical removal of vegetative microbes.

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12
Q

What is a germicide?

A

A substance or treatment capable of destroying microbes.

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13
Q

What are germistatic compounds?

A

Substances capable of inhibiting microbial growth (but not killing them).

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14
Q

What is one of the most common methods of destroying microbes?

A

Heat.

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15
Q

How do high temperatures affect microbial cells?

A

Denature proteins in microbial cells, leading to cell death.

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16
Q

Name three methods of heat sterilization.

A
  • Moist heat sterilization
  • Dry heat sterilization
  • Pasteurization
17
Q

What effect do low temperatures have on microbes?

A

Often have a germistatic effect.

18
Q

What is pasteurization?

A

Mild heating methods to kill pathogenic or food-spoilage bacteria.

19
Q

Who first described pasteurization?

A

Louis Pasteur.

20
Q

What is the primary use of filtration?

A

To remove microbes from liquids or gases.

21
Q

What determines what can pass through a filter?

A

The size of the pores.

22
Q

What is non-ionizing radiation?

23
Q

What is ionizing radiation?

A

X-rays, gamma rays.

24
Q

What is desiccation?

A

The removal of water from organic matter.

25
What effect does desiccation primarily have?
Germistatic effects.
26
What are chemical agents used for in microbial control?
Sanitization and disinfection.
27
What is the use-dilution test?
A method for evaluating the effectiveness of disinfectants.
28
What do phenols and phenolics do?
Disrupt cell membranes by interacting with lipid molecules.
29
What effect does iodine have?
Interferes with protein synthesis.
30
How do alcohols function as disinfectants?
Effective against bacteria, fungi, and enveloped viruses.
31
What do compounds of heavy metals do?
Denature essential proteins in cellular metabolism and function.
32
What are surface-active agents also known as?
Surfactants.
33
What do soaps and detergents primarily accomplish?
Degermation.
34
What is the function of acid-anionic sanitizers?
Disrupt the plasma membrane in a high-acid environment.
35
What are quaternary ammonium compounds (quats) ineffective in?
The presence of organic molecules.
36
How do chemical food preservatives work?
Inhibit mold growth by interfering with metabolism or membrane integrity.
37
What are aldehydes known for?
Very effective disinfectants due to their protein denaturation activity.
38
What does chemical sterilization do?
Inhibits cellular functions in microbes.
39
What do peroxygens and other forms of oxygen do?
Use oxidation to disrupt chemical bonds in microbes.