Micro Flashcards
Purpose of peptidoglycans
gives rigid support, protects against osmotic pressure, determines how much gram strain will be picked up. Sugar backbone with peptide side chain cross linked by transpeptidase
Gram + vs gram - color
Gram + pruple, gram - pink
function of cell wall/cell membrane (gram +)
major surface antigen, induces TNF and IL1
function of outer membrane (gram -)
site of endotoxin, lipid A induces TNF and IL-1
periplasm function
space btwn cytoplasmic membrane and outer membrane in gram negative bactr, contains hydrolytic enzymes including B lactamases
glycocalyx function
mediates adherence to surfaces, especially foreign substances – sugar coat, think biofilm/capsule
Gram + bacteria examples
- Staph, strep (cocci)
- clostridium, corynebacterium, bacillus, listeria, mycobacterium,gardnerella (bacillus)
- actinomyces, nocardia (branching)
Gram negative examples
-neisseria (coccus)
==RODS==
- Ecoli, shigella, salmonella, yersinia, klebsiella, proteus,enterobacter,serratia, bivrio, campylobacter,helicobacte,r psuedomonas, bacteriodes(enterics)
- haemophilus, legionella, bordetella (respiratory)
- francisella, brucella, pasteurella, bartonella (zoonotic)
- leptospira, borrelia, treponema (spirochete)
- ricketssiea,chlamydia (plenomorphic)
Unique thing about cell membrane/wall for mycoplasma
contains sterols and has no cell wall (hence why some abx dont work)
Unique thing about mycobacteria cell wall
contain mycolic acid,high lipid content
Capsule function in bacteria
protects against phagocytosis,usually polysaccharide (except D-glutamate in bacillus anthracis)
Bugs which dont gram stain well
“These Rascals May Microscopically Lack Color”
-Treponema, Rickettsia, Mycobacteria, Mycoplasma, Legionella, Chlaymdia
can use silver stain for legionella
for waht bugs do you use Giemsa stains
Chlamydia, Borrelia,Rickettsiae, Trypanosomes, Plasmodium,Histoplasm
“Certian Bugs Really Try His Patience”
PAS stains for..
Stains glycogen/mucopollysacharides –> Tropheryma whipplei
Ziehl Neelson stain
Acid Fast organisms (nocardia, mycobacteria)
India Ink stain
cryptococcus neoformans
Bug grown on Chocolate agar w Factors V (NAD+) and X (hematin)
H. Influenza
Culture reqs for H Infleunza
Chocolate Agar w/ FV and FX (NAD+ and hematin, respectively)
Thayer martin media grows what two bugs, and has what 3 bugs
Neissseria gonorrhoea and neisoerria meningitidis
VPN: Vancomycin, polymyxin, nystatin
Bordet-Gengou (potato) agar grows
Bordetella
Loffler’s Media grows
C Diphtheriae
Lowenstein Jensen agar grows
TB
Eatons agar grows
M pneumoniae
MacConkey’s agar grows, and what color do fermenting bac grow
they turn pink; Think lactose fermenting bacteria (Citrobacter, Ecoli,klebsiella, enterobacter, serratia)
CKEES
Charcoal yeast extract grows
Legionella
Sabarouds agar grows
Fungi
“Sabaroud is a fun guy”
Obligate aerobes include
Are O2 depedent, and includes
Nocardia, pseudomonas, mycobacterum tberculosis, bacillus
“Nagging Pests Must Breathe”
Obligate anaerobes include
typical abx include?
Clostridium, bacteriodes, actinomyces
they lack catalose/superoxide dismutase –> are susceptible to oxidative damage, genreally foul smeling
Tx: clindamycin, metronidazole
“can’t breathe air”
Obligate intracellular bugs
Rickettsia and Chlamydia (cant make their own ATP)
Facultative Intracellular
Salmonella, Neisseria, Brucella, Mycobacterium, Listeria,Francisella, Legionella, Yersinia estis
“Some Nasty Bugs May Live FacultativeLY”
Encapsulated Bacteria have what + reaction, and include what species?
+ quellung reaction
Strep pneumo, Haemophils influenza, Neisseria Meningitids, E Coli, Salmonella, Klebsiella, GBS, Pseudomonas
“Even Some Pretty Nasty Killers Have Shiny Bodies”
Quellung reaction indicates what?
Encapsulated species
Catalase + organisms include
Also, pts with what disease are more prone to these infections?
Pseudomonas, Listeria, Aspergillus, Candidda, EColi, Staph Aureus, Serratia
Pts with chronic granulomatous disease have recurrent infxns
Pigment of the following bacteria:
- Staph Aureus
- Actinomycess Israelii
- Serratia Marcescens
- Pseudomonas Aeurginosa
- gold
- gold
- red
- green
Importance of bacterial viurlence factor Protein A, which bug has it?
Binds Fc region of Ig, prevents opsonization and phagocytosis – staph aureus
Importance of bacteriaul virulence factor - IgA protease, who has it?
Enzyme cleaves IgA, Strep Pneumo, H Influenza, Neisseria (SHiN)
Importance of bacterial virulence factor M protein - who has it
prevents phagocytosis, seen on Group A strep
More toxic: endo or exotoxin?
Exotoxin
Result of Endotoxin
- Activates macrophages (IL1, TNF, and NO release)
- Activates complement (C3a, C5a)
- Activates tissue factor (DIC)
ENDOTOXIN:
Edema, NO, DIC/Death, Outer Membrane, TNFa, O-antigen, eXtremely heat stable, IL-1, Neutrophil chemotaxis
Toxin associated with corynebacterium diptheria and MOA
Diptheria toxin, inactivates elongation factor 2
Pseudomonas aeruginosa toxin and MOA
Exotoxin A - inacitvates elongation factor 2
Shigella toxin and MOA
shiga toxin - inactivates 60S ribosome by removing adenine from rRNA - causes GI mucosal damage –> dysentery
EHEC toxin and moa
Shiga Like Toxin (verocytotoxin) which inactivates 60S ribosome by removing adenine from rRNA
two toxins of ETEC and MOA
increased fluid secretion
LT and ST (heat labile and heat stable)
- LT activates adenylate cyclase, leading to increased cAMP –> increase Cl secretion in gut and H2o efflux)
- ST activates guanylyl cyclase,leading to increased cGAMP –> decreased NaCl and H2o resoption in gut
Wattery diarrhea: labile in the Air (cAMP) and stabile on the ground (cGMP)
Bacillus anthracis 3 toxins and MOA
edema factor, lethal factor, protective Ag
mimics adeylate cylcase activity (increased cAMP), causes increased fluid secretion
vibrio cholera releases..
cholera toxin, overactivates adenylate cycalse (cAMP) by permanently activating Gs –> incnreased CL- and H2O secretion in gut
Bodetella pertussis toxin and activity
What disease is caused?
Pertussis toxin which activates adenylate cycalse –> increased cAMP, impairs phagytosis
causes whooping cough
Clotridium tetanus rleases what toxin, and what MOA?
Tetanospasmin, cleaves SNARE protein required for NTM release, prevents release of inhibitory (GABA and glycine) NTM,causing muscle rigidity
Clostriduum botuliniuum has what exotoxins
Botulinum toxin, cleaves SNARE protein required for NTM release, prevents release of stimulatory (ACh) signal at NMJ, causes flaccid paralysis
Yersinia Enteridis toxin, associated with what disease?
similar to ETEC- overactivates cGMP, causes abdpominal pain, “psuedo appendicitis”
Clostridium perfringens releases what two toxins and has what MOA
releases alpha toxin, which is a phospholipase which degrades tissue and cell membranes.
also has enterotoxin for food poisoning
Strep pyogenes exotoxin
Streptolysin O, which degades cell membranes
Staph Aureus Toxins (x2?)
TSST-1 and Enterotoxins
TSST brings MHC II and TCR in proximity, causing overwhelming release of IFNy and IL-2 –> shock
Strep pyogenes (GAS) has what superantigen and what MOA?
Exotoxin A –> brings MHC II and TCR in proximity, causing overwhelming release of IFNy and IL-2 –> shock
Meaning of alpha vs beta vs gamma hemolysis
Alpha - partial hemolysis with green cleaning (strep pneumo or strep viridans)
Beta - completel hemolysis with white clearing (GAS) - Strep pyogenes or GBS (Strep agalictiae)
Gamma - no hemolysis, no clearining - Enterococcus
Clinical presentation of Strep Pneumo
Meningitis, Otitis Media, Pneumonia, Sinusitis
MOPS
Staph exotoxins can cause what?
Shock, Staph Scalded skin Syndrome (TSST)
gastroenteritis (enterotoxin)
Rheumatic Fever Criteria?
JONES
- Joint pains
- Pancarditis
- Nodules
- Erythema marginatum
- Sydenham’s Chorea
Toxins associated with Strep Pyogenes (GAS)
- Streptlysin O –> hemolysis, O2 labile
- Streptolysin S - hemolysis, O2 stable
- Eryhtogenic/Pyogenic toxins –> Skin rash, fever, scarlet fever
- Exotoxin : toxic shock like syndrome
Pyogenic, Toxigenic, and Immunologic result of GAS/Strep Pyogenes
- pharyngitis, cellulitis, impetigo
- Scarletfever, toxic shock like syndrome, necrotizing fasciitis
- PSGN, Rheumatic Fever
causes of cellulitis/impetigo
Staph aureus and strep pyogenes
Clostridium difficile’s two toxins MOA
Often secondary to what abx use?
Toxin A: enterotoxin binds to brush border of gut
Toxin B: cyotoxin, destroys the cytoskeletal structure of enterocytes, causing psuedomembranous colitis
Usually clindamycin, ampicillin
Waterhouse friderichsen syndrome? caused by?
Adrenal hemorrhage, casued by N. Meningococcus
Hemophiluscuases what sx?
hEMOPhilus
Epiglottis, meningitis, otitis media/sinusitis, pneumonia