Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

CSMLS 2024 > Micro > Flashcards

Micro Flashcards

1
Q

what is MRSA known to be resistant to

A

beta lactam antibiotics

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

what antibiotics is susceptable to MRSA

A

vancomyosin or linezolid

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Common antibiotic for strep pneumo

A

penicillin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

common antibiotic for enterococcus faecalis

A

ampicillin and vancomycin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

what is CNA agar selective for

A

GPO

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

alpha hemolysis principle

A

partial breakdown of haemoglobin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

beta hem principle

A

complete breadown on haemoglobin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

gamma hem principle

A

bacteria are non hemolytic

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

principle and purpose of catalase test

A

enzyme that converts hydrogen peroxide into water and oxygen

differentiates strep (neg) and staph (pos)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

false pos of catalase

A

rbc carry over
enterococci

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

false neg of catalase

A

old colonies

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

principle and purpose of coagulase

A

converts fibrinogen to fibrin using rabbit plasma on a BAP plate to detect staph aureus (pos)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

false pos of coagulase test

A

staph isolated on selective media

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

principle and purpose of DNAse

A

DNAse enzyme cleaves DNA to leave a colorless media

differentiate of aureus (pos) and micrococcus (neg)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

what is the gold standard of MRSA ID

A

PCR - but using Oxacillin disc test works

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

location and cause of strep pyo (GAS)

A

pharyngitis causing strep throat

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

location and cause of strep agal (GBS)

A

gential tract causing neonatal sepsis and meningitis

18
Q

location and cause of strep pneumo

A

URT fllora causing pneumonia, eye and ear infection

19
Q

location and cause of viridian strep

A

mouth and URT flora causing heart damage

20
Q

location and cause of enterococci

A

GI flora causing UTI and wound infections

21
Q

what antibiotic can enterococci become resistant to

A

vancymycin resistancve enterococci (VRE)

22
Q

what does a pos PYR look like

A

red dot on poked disc= pos

23
Q

what is bacitracin (Taxos A) positive result

A

any zone of inhibition seen

24
Q

how to set up a CAMP test

A

S aureus middle line, then perpendicular lines to the middle line

25
Q

what is a pos CAMP test look like and what organism

A

arrow heads as GBS is pos

26
Q

what does a pos BEA look like

A

black coloration

27
Q

what does pos NaCl tolerance test look like

A

purple to green = pos
BUT if no color change and BAP grew, then still pos

28
Q

principle of decarboxylase

A

medium contains glucose and bromcresol purple. tubes will turn yellow (acidic) incubated due to glucose fermentation. tubes revert to purple (basic) if decarboxylation occurs.

29
Q

what color should the control tubes for decardboxylase be

A

yellow - we want to make sure glucose ferments and its the bacteria that decarboxylases.

30
Q

Common characteristic of enterobateriaceae

A

GNB
- ferment glucose
- nitrate to nitrITES
- ox neg
-non spores

31
Q

principle of cytochrome oxidase test

A

TMPD interacts with the ETC of cytochrome C making dark purple indophenol. (interacts w bacteria containing cytochrome C)

32
Q

false negative of oxidase test

A

selected off MAC - use only fresh BAP 12-24 hrs

33
Q

principle of TSI

A

carb fermentation (glucose, lactose, sucrose)
H2S production
gas production

34
Q

what are non fermenters TSI

A

K/K (red)

35
Q

what are non lactose fermenter TSI

A

K/A

red/yellow - glucose fermented but not lactose

36
Q

critrate principle and pos

A

turns blue = pos due to alkaline products produced

37
Q

indole principle and pos

A

hydrolyze triptophan to form indole which is detected using kovacs

cherry red = pos

38
Q

malonate pos colour

A

change of blue to green = pos

39
Q

phenylalanine test pos

A

add ferric chloride - green = pos

40
Q

urease test pos

A

cherry red = pos

41
Q

OF test principle and pos

A

aerobic = oxidation to gluconic acid

anaerobic = fermentation to triose molecules

yellow = pos
blue = neg (gluose not used)

42
Q
A

CSMLS 2024 (3 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions