MHC genetics

Question 1

What TFs make up the SXY module? Enhancersome?

Answer 1

RFX CREB NF-Y

Question 2

What binds to the SXy enhancesome?

Answer 2

The CIITA/ CITA

Question 3

How does CIITA regulate MHC II?

Answer 3

Works in a cell-type specific manner to regulate MHCII via chromatin, transcriptional initiation and elongation.

Question 4

What JAKs and STATs are mporatn for IFN-y asiganlling?

Answer 4

JAK 1 and JAK2 and STAT1.

Question 5

How does STAT1 upregualte CIITA?

Answer 5

STAT1 binds to GAS to promote the expression of IRF1 which binds to ISRE to upregualte CIITA/

Question 6

What is another name for the CITA?

Answer 6

NLRC5

Question 7

What signalling pathway binds GAS to upregulate NLRC5 transcription? How does NLCR become active?

Answer 7

IFN-y and JAK1/2 STAT1 pathway binds GAS to upregulate transcription of NLRC5.
NTP binds inactive NLRC5 to activate it.

Question 8

naming of MHC alleles?

Answer 8

serological, protein level, substitutions withn coding region, substitutions in non coding regions.

Question 9

What HLA-B allele was associated with protection in malaria ?

Answer 9

HLA-B53 protective against malaria.

Question 10

Interesting about the HLA-B53 binding groove?

Answer 10

it has a widened groove that binds with water to provide functional malleability- bound epitopes with high specificity that had low affitnity.

Question 11

Protective HIV HLA?

Answer 11

HLA-B57, although it may haev evolved to avoid this.

Question 12

what HLA was associated with abacavir sensititivy?

Answer 12

HLA; B57:01.

Question 13

Why does HLA-B57:01 cause hypersensitivity?

Answer 13

abacavir can sit in grrove and allows slef peptides to be presented to CD8+ cells.