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SBB > Methods > Flashcards

Methods Flashcards

1
Q

LISS method of enhancement

A

decreases ionic strength

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2
Q

PEG method of enhancement

A

removing water and concentrating antibody

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3
Q

Glycoprotein blood groups

A

HLA system

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4
Q

Glycolipid blood groups

A

ABH, Lewis, Ii, and P systems

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5
Q

Protein blood groups

A

Rh, M, N

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6
Q

greatest to least amount of H in blood groups

A

O > A2 > B > A2B > A1 > A1B

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7
Q

What does Dolichos biflorus aggluntinate?

A

A1 or A1B cells

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8
Q

What does Bandeiraea simplicifolia agglutinate?

A

B cells

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9
Q

What does Ulex eurpoaeus agglutinate?

A

O cells/H-antigen (anti-H)

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10
Q

ABO antigens can be detected on red cells of embryos as early as:

A

5 to 6 weeks of gestation

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11
Q

adult levels of ABO antigens are generally observed by age

A

2 - 4 y/o

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12
Q

FUT1

A

H gene

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13
Q

FUT# that specifically fucosylates type 2 chain oligosaccharides on red cell glycoproteins and glycolipids to form type 2 chain H

A

FUT1

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14
Q

FUT2

A

Secretor gene

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15
Q

FUT# that recognizes type 1 chain precursors to form type 1 chain H and Leb antigens

A

FUT2

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16
Q

FUT3

A

Lewis gene

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17
Q

R1

A

DCe

40% W, 17% B, 70% A

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18
Q

R2

A

DcE

15%

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19
Q

R0

A

Dce

5% W, 45%B

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20
Q

RZ

A

DCE

<0.01%

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21
Q

r

A

ce

35% W, 25% B

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22
Q

r’

A

Ce

2%

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23
Q

r’’

A

cE

1% W, <0.01%B

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24
Q

ry

A

CE

<0.01%

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25
Q

Rh1 ISBT

A

D

85 W, 92B

26
Q

Rh2 ISBT

A

C

70 W, 30 B

27
Q

Rh3 ISBT

A

E

30%

28
Q

Rh4 ISBT

A

c (little)

80 W, 95 B

29
Q

Rh5 ISBT

A

e

98%

30
Q

Rh6 ISBT

A

ce or f

65 W, 27B

31
Q

Rh7 ISBT

A

Ce or rh(i)

70 W, 30B

32
Q

Rh8 ISBT

A

Cw

low, 2%W

33
Q

Rh9 ISBT

A

Cx

low

34
Q

Rh10 ISBT

A

V

30% B

35
Q

Rh11 ISBT

A

Ew

low

36
Q

Rh12 ISBT

A

G

85%

37
Q

Strength of D (haplotypes in order)

A

R2R2, R1R2, R2r, R1r

38
Q

A-subgroup that shows mixed field in forward type

A

A3

39
Q

A-subgroup that shows reactivity with Anti-A,B, typically no reactivity with Anti-A

A

Ax

40
Q

B subgroup that shows mixed field in forward type

A

B3

41
Q

B subgroup that shows 0/wk with Anti-B and 0/2+ with Anti-A,B

A

Bx

42
Q

K KEL1

A

9 W, 2 B

43
Q

k KEL2

A

99%

44
Q

Kpa KEL3

A

2 W, <0.01 B

45
Q

Kpb KEL4

A

100%

46
Q

Ku KEL5

A

100

47
Q

Jsa KEL6

A

0.01 W, 20 B

48
Q

Jsb KEL7

A

99%

49
Q

IgM % of total serum concentration

A

5-10%

50
Q

IgG % of total serum concentration

A

80%

51
Q

IgM serum half-life

A

5-6 days

52
Q

IgG serum half-life

A

23 days

53
Q

apheresis donor rxn: chills, parasthesias, nausea/vomiting, cramping, cardiac arrhthymia

A

citrate reaction

54
Q

apheresis donor rxn: respiratory distress, chest pain, collapse

A

air embolism

55
Q

apheresis donor rxn: flushing, hives, cough, respiratory distress

A

anaphylactic reaction

56
Q

apheresis donor rxn: back pain, shock, cardiac arrest

A

hemolysis

57
Q

RBC life span

A

120 days

58
Q

granulocytes life span

A

half life = 7.2 hours

59
Q

lymphocytes life span

A

4 - 10 years

60
Q

monocytes life span

A

half life = 71 hours

61
Q

platelets life span

A

8-10 days

SBB (3 decks)

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