Methods Flashcards
1
Q
Total serum protein
A
Biuret reagent
Copper ions bind with protein
2
Q
CSF protein
A
Turbidometric using Nephlometry (scattered light)
3
Q
Serum albumin
A
Bromocresol green
4
Q
Urinary albumin excretion
A
Albumin/creatinine ratio
A high urinary albumin excretion might mean renal failure in diabetics
5
Q
LD (enzyme)
A
Reverse rxn using pyruvate, NAD and NADH
6
Q
CK
A
Reverse rxn using creatine Po4 substrate
7
Q
ALT
A
Alanine reaction
8
Q
AST
A
Asparte rxn
9
Q
ALP
A
4NPP
10
Q
Amylase
A
Malto-tetraose
11
Q
Lipase-enzymatic
A
Triglycerides»_space; chromagen
12
Q
GGT
A
y glutamyl p-nitroanilide
13
Q
Tumor markers
A
Sandwich immunoassay (enzyme, fluorescent, chemiluminescent)
MAINLY ELISA.
14
Q
Ideal specimen of choice for tumor markers
A
Serum or heparin
Fridge <7 days
Frozen <4 weeks
avoid freeze/thaw
15
Q
Urea/Urease
A
Berthelot rxn of NH3
