Methane Oxidation Flashcards
What organisms do methane oxidation?
methanotrophs
What enzymes are involved in methane oxidation?
particulate methane monooxygenase
water soluble monooxygenase
Which genes are involved in methane oxidation?
pmoA
mmoX
What types of organisms are methanotrophs?
Type I Gammaproteobacteria
Type II alpha proteobacteria
What are methanotrophic bacteria a subset of?
methylotrophic bacteria
what are methylotrophic bacteria?
aerobic bacteria that use one-carbon compounds more reduced than formic acid as a source of carbon and energy like methanol, methylamines, halomethane
What is the net reaction for methane oxidation?
CH4 + O2 + ADP + Pi = CO2 + ATP
What are methanotrophs?
can use methane or methanol as their ability to use methane as their sole carbon and energy source
Does methane oxidation require energy input?
yes
What catalyzes the first step in methane oxidation?
methane monooxygenase
What does methane monooxygenase require to initiate methane oxidation?
electrons from cytochrome c in the respiratory ETC produced by NADH
What are the steps of methane oxidation?
CH4 > CH3OH > CH2O (formealdehyde) > HCOO- (formic acid) > CO2
What is the product of methane oxidation?
CO2 + energy (ATP)
How does methane oxidation interact with the electron transport chain in respiration?
methane monooxygenase requires electrons provided by cyt c produced by NADH in the ETC
other steps of oxidation of CH3OH to CO2 provide electrons to the ETC
What pathway do type I methanotrophs (gamma proteobacteria) follow?
RuMP pathway
