METALLIC FIXATIVES Flashcards
most common metallic fixative
mercuric chloride
mercuric chloride fixatives may produce black granular deposits excepts
heidenhain susa
removal of black granular deposits can be done by ______
dezenkerization (washing out)
pentrates and hardens tissue rapidly
mercuric chloride
routine fixative of choice for preservation of cell detail in tissue photography
mercuric chloride
fixative of choice for hematopoietic and reticuloendothelial tissues (for bone marrow spx)
mercuric chloride
recommended for small pieces of liver, spleen, CT fibers, and nuclei
Zenker’s: contain Glacial Acetic Acid
recommended for Trichrome Staining
Zenker’s: contain Glacial Acetic Acid
contains potassium dichromate and formalin
Zenker’s formol (AKA Helly’s/Kelly’s)
excellent microanatomical fixative of pituitary gland, BM, and blood containing organs
Zenker’s formol (AKA Helly’s/Kelly’s)
preserves cytoplasmic granules
Zenker’s formol (AKA Helly’s/Kelly’s)
used to remove brown pigments in Zenker’s formol (AKA Helly’s/Kelly’s)
picric acid/NaOH
has TCA, Glacial Acetic Acid, Formalin
Heidenhain susa
recommended for skin tumor biopsies
heidenhain susa
anhydrous sodium acetate
B5 fixative
bone marrow biopsies; lymph nodes
B5 Fixative
mercuric chloride fixatives
- Zenker’s contain glacial acetic acid
- Zenker’s Formol (Helly’s/ Kelly’s
- Heidenhain susa
- B5 fixative
strong oxidizing agents and should not be combines with reducing agents
chromate fixatives
preserves carbohydrates
chromate fixatives
preserves lipids and mitochondria at pH 4.5-5.2 cytoplasm, chromatin, and chromosomes are fixed
potassium dichromate
fixative for chromatin, mitochondria, mitotic figures, golgi bodies, RBC and colloid containing tissues
Regaud’s (Muller’s)
for early degenerative processes and tissue necrosis
Orth’s Fluid
demonstration of rickettsia; preserves myelin
orth’s fluid
4% aqueous solution and recommended for acid mucopolysaccharides
Lead fixatives
