ALCOHOL FIXATIVES Flashcards
rapidly denatures and precipitates proteins
alcohol fixatives
acts as fixative and dehydrating agent
alcohol fixatives
alcohol fixatives preserves nuclear stains but dissolves ________
fats and lipids
for dry and wet smear (blood and bone marrow smears)
methanol
disadvantage of methanol
toxic when ingested
other term fomethanol alcohol
wood alcohol
causes of ingested alcohol fixatives
blindness/death
fixes touch preparations
isopropyl alcohol
stain to used in methanol fixative
Wright Giemsa stain
other term for isopropyl alcohol
rubbing alcohol
simple fixative; doesn’t fix glycogen; useful for PCR
ethanol
most rapid fixative
Carnoy’s fixative
fixes and dehydrates at the same tome
carnoy’s fluid
fixes Nissl granules and cytoplasmic granules
carney’s Fixative
recommended for chromosomes, lymph glands, and urgent biospis
Carnoy’s fixative
recommended for mucopolysaccradies and nuclear
Newcomer’s
acts as both nuclear and biochemical fixative
Newcomer’s
acts as both a nuclei, and biochemical fixatives
newcomer’s
