Metallic Fixatives Flashcards
1. mercuric chloride 2. chromate fixatives 3. lead fixatives 4. picric acids
permits brilliant staining of nuclear and connective tissue fibers
Zenker’s Solution
fixes connective tissue mucin
lead fixatives
good fixative for connective tissue, excellent for preserving glycogen, and extracts, CHONS lipids
picric acid
for preserving soft and delicate structures (e.g., endometrial curettings).
Gendre’s solution
for bone marrow, extramedullary hematopoiesis and intercalated discs of cardiac muscle
Helly’s Solution (zenker-formol)
provides superior results in immunostaining of biogenic and polypeptide hormones
picric acid
Chromic Acid used in what percent solution
1-2% aqueous solution
recommended for the demonstration of chromatin, mitochondria, mitotic figures, Golgi bodies, RBC and colloidcontaining tissues.
Regaud’s (Muller’s) Fluid
recommended for trichrome staining
Zenker’s Solution
tissues fixed with mercuric chloride containing compounds produce black precipitates except?
Haidenhein’s susa
fixation of hematopoietic, bone marrow biopsies and lymphoid tissue, commonly used in bone marrow biopsies
Lillie’s B-5 Fixative
Potassium Dichromate used in what percent solution
3% aqueous solution
for congested specimens
Zenker’s Solution
may act as a mordant for special staining techniques
Zenker’s Solution
precipitates all proteins and adequately preserves carbohydrates
Chromic Acid