Aldehyde Fixatives Flashcards
percent of formalin that is most widely used
10% formalin
for central nervous tissue and general post-mortem tissues for histochemical examination
10% formal saline
formaldehyde should be changed for every?
3 months
demonstrates fats and mucin
10% formol saline
Best general tissue fixative
10% Neutral-Buffered Formalin
karnovksy’s fixative composed of?
parformaldehyde + glutaraldehyde
best fixative for tissues containing iron pigments and for elastic fibers
10% Neutral-Buffered Formalin
excellent medium in which to store whole blocks of tissue
formalin sodium acetate solution
recommended for the preservation of lipids, especially phospholipid
10% formal saline
excellent for many staining procedures including silver reticulum methods
Formol-Corrosive
nuclear studies of bacteria
zenker’s formalin solution
recommended for surgical, post-mortem and research specimen
10% neutral buffered formalin
use in rapid diagnosis, fix sputum good for glycogen
formalin alcohol solutin/gendres
for light microscopy in resin embedding and section for electron microscopy
karnovksy’s fixative
Nuclear Fixatives
Bouin’s fluid
Flemming’s fluid
Newcomer’s fluid
Carnoy’s fluid
Heidenhain’s Susa
excellent fixative for liver, spleen, connective tissue fibers and nuclei
zenker’s formalin solution
preserves enzymes and nucleoproteins
10% formal saline
most commonly used fixative
formaldehyde
best fixative for frozen section
10% Neutral-Buffered Formalin
Histochemical Fixatives
Formal Saline 10%
Absolute Ethyl Alcohol
Newcomer’s Fluid
Acetone
Cytoplasmic Fixatives
Helly’s fluid
Orth ‘s fluid
Regaud ‘s fluid (Muller ‘s fluid)
Flemming’s fluid without acetic acid
Formalin with “post-chroming”
paraformaldehyde is formed due to?
white powders deposits formed due to long storage in cold place
ideal for most staining techniques, including silver impregnation
10% formal saline
how can the formation of paraformaldehyde retarded
addition of 10% methanol to formalin solution
