Metabolic assays (Lab) Flashcards
What is a starch hydrolysis test used for?
To test for the presence of amylase and starch hydrolysis
What is an oxidative fermentation test used for?
To test if the organism is using oxidation or fermentation to metabolize glucose
What is a carbohydrate fermentation test used for?
If an organism can use a particular carbohydrate as an energy and carbon source
What is a methyl red (MR) test used for?
Tests if an organism is doing mixed acid fermentation
What is a Voges-Proskauer test used for?
Tests if an organism is doing butanediol fermentation
What is a citrate test used for?
Tests for the presence of citrate lyase and if the organism can use citrate for fermentation
What is a KOH test used for?
Confirms if an organism is gram-positive or gram-negative
What is an oxidase test used for?
Tests for the presence of cytochrome C
What is an ONPG test used for?
Tests for the presence of beta-galactosidase and lactose breakdown
What is an indole test used for?
Tests for the presence of tryptophanase and indole production
What is MacConkey agar used for?
Selects for enteric gram-negative bacteria and differentiates them based on lactose fermentation
What is EMB agar used for?
Selects for gram-negative bacteria and differentiates them based on fermentation type
What is a catalase test used for?
Detects the presence of catalase to differentiate between the Micrococcaceae and the Streptococcaceae
What is a nitrate reduction test used for?
Tests for the ability of an organism to do nitrate reduction
What is blood agar used for?
Used to grow fastidious microbes and tests for hemolysis and the production of hemolysins