Medias Flashcards
as differential: allows differentiation of hemolysis
tryptic soy agar with 5% sheep blood
sheep blood agar
as enrichment: supplies X (hemin) and V (NAD) factor
CHOC
medias selective to gram (+)
Colistin and Nalidixic Agar (CNA) + Phenylethyl Alcohol agar (PEA)
use for culturing streptococci, pneumococci, and meningococci
Brain Heart Infusion Agar
as selective: isolates enteric gram (-) rods
as differential: lactose vs non-lactose fermenters
Eosin and Methylene Blue (EMB), MAC, and XLD agar
color of lactose fermenters in EMB media
green-black or purple
E. coli: green metallic sheen
color of lactose fermenters in MAC media
pink
selective media for E. coli 0157:H7
Sorbitol MacConkey agar (SMAC)
E. coli 0157:H7 doesn’t ferment sorbitol = colorless!
pH indicator of MAC and salmonella-shigella agar
neutral red
inhibitory component of MAC
inhibits g (+)
bile salts and crystal violet
pH indicator of Hektoen-Enteric Agar and Hugh-Leifson OF medium
bromthymol blue
agars that have phenol red as indicator of pH
XLD, MSA, and TSI agar
sugars present in XLD agar
- lactose
- sucrose
- xylose
sugars present in HEA
- lactose
- sucrose
- salicin
inhibitory component of XLD agar
sodium deoxycholate
appearance of Shigella and Salmonella on HEA
green colonies
but Salmonella has black center
E. coli on HEA and XLD
yellow colonies
enrichment media for Campylobacter
Thioglycolate medium
incubation at 4℃
transportation and growth media of Neisseria gonorrhoeae
JEMBEC plates
differential medium for isolation of C. diphteriae
Cystine-tellurite blood agar and Tinsdale agar
enrichment medium for C. diphtheriae
Loeffler medium
promotes development of metachromatic granules
selective for Salmonella only!
Bismuth sulfite agar
selective for Yersinia, Aeromonas, and P. shigelloides
Cefsulodin-Irgasan-Novobiocin (CIN) agar
appears as red “bull’s eye” colonies surrounded by colorless halo on CIN agar
Y. enterocolitica
enrichment for Vibrio spp.
Alkaline Peptone Water
selective for Vibrio spp
TCBS agar
yellow colonies on TCBS agar
ferments sucrose
V. cholerae
appears like mercury droplets in Bordet-Gengou agar
Bordetella
potato-glycerol-based medium enriched with blood
selective enrichment medium for B. pertussis
Bordet-Gengou agar
charcoal agar with horse blood, cephalexin, and amphotericin B
selective for B. pertussis
Regan-Lowe agar
enrichment for Legionella
has yeast extract and L-cysteine
BCYE agar
component of Martin-Lewis that inhibits growth of yeast/mold
Anisomycin
