med sig Flashcards
- catalase positive, grow in BAP as creamy, white, pinhead colonies
- Grows in MSA and ferments mannitol (yellow colonies)
S. aureus
- Grows in MSA but cannot ferment mannitol
- susceptible to novobiocin
- bacterial endocarditis after prosthetic heart valve insertion
S. epidermidis
- resistant to novobiocin
- UTI among sexually active young females
S. saprophyticus
- PYRase positive
- sensitive to 0.04U Bacitracin (Taxo A)
- Sulfamethoxazole-Trimethoprin resistant
S. pyogenes
- Beta hemolytic in BAP
- CAMP (Christie-Atkins-Munch-Peterson) positive
- Hippurate hydrolysis positive
- Neonatal meningitis
S. agalactiae
- (+) bile esculin test
- (+) 6.5% salt tolerance test
- (+) PYRase
- grows at 10℃ and at 45℃
Enterococcus
Group D
- lancet or bullet shaped
- Alpha hemolytic
- Lobar pneumonia and meningitis
S. pneumoniae
(+) Mouse inoculation test
(+) inulin fermentation
(+) bile solubility test
(+) optochin susceptibility
(+) Neufeld Quellung test positive
S. pneumoniae
dental carries and subacute bacterial endocarditis
Viridans
(-) Mouse inoculation test
(-) inulin fermentation
(-) bile solubility test
(-) optochin susceptibility
(-) Neufeld Quellung test positive
Viridans
What does nutritionally variant streptococci requires?
vitamin B6 (pyridoxal) and thiol (cysteine)
What causes opthalmia neonatorium?
N. gonorrhoeae
Carbohydrate utilization test result: uses glucose
N. gonorrhoeae
Waterhouse Friedrichsen Syndrome
N. meningitidis
morphologically similar to Neisseria but asaccharolytic and DNAse positive
Moraxella catarrhalis
pseudomembrane in throat
C. diphtheriae
assoc. with spontaneous abortion
L. monocytogenes
- tumbling end-over-end motility
umbrella-shaped or inverted Christmas tree pattern
(+) Bile esculin hydrolysis
(+) Catalase
(+) CAMP test
(+) Voges-Proskauer positive
L. monocytogenes
specific test for L. monocytogenes
Ocular test of Anton
- butcher’s disease
- seal finger
- whale finger
- erysipeloid
E. rhusiopathiae
long, thin, branching, gram positive bacilli; partially acid fast
Nocardia
- Grows in MacConkey and EMB
- TSIA = A/A with gas
- IMVC ++00
- LOA ++0
- motile
E. coli
- Growns in MacConkey
- TSIA = A/A with gas
- Non motile
- IMVC 00++
- LOA +00
- weakly urease positive
K. pneumoniae
What test differentiates K. pneumoniae and K. oxytoca?
K. oxytoca is indole (+)
- Produces red pigment prodigiosin; agent of red milk
- Lipase positive, gelatinase positive, DNase positive
DNase is what differentiates it from other Enterobac
Serratia marcesens
- IMVC ++0
- H2S positive
- LOA ++0
- TSIA = K/A
Edwardsiella tarda
specific test for Salmonella
Widal test
- Resembles Salmonella
- (+) ONPG positive
- (-) Lysine decarboxylase
Citrobacter spp.
- Biologically inert
- non-motile
- bacillary dysentery
- Grows in MacConkey and HEA
- TSI = K/A
Shigella
- TSIA = K/A with gas and H2S
- Motile
- (+) phenylalanine deaminase
- strongly urease positive
Proteus spp.
- Associated with plague - transmitted by flea
- stalactite patterns; stained using Wayson stain
Yersinia pestis
Red bull’s eye colonies in CIN
Y. enterocolitica
- Grape-like odor, feather edges, produces pyocyanin and pyoverdin
- motile, grows at 35 and 42℃
- TSIA = K/K
- (+) oxidase
- (+) acetamide utilitzation
- (+) cetrimide positive
Pseudomonas aeruginosa
Glander’s disease
B. mallei
melioidosis
B. pseudomallei
- pink eye conjunctivitis
- requires X and V factor
H. aegyptius
- soft chancres
- schools of fish in Gram stain
- requires X factor only
H. ducreyi
- Pitting of agar
- bleach like odor
Eikenella corodens
- Cultured in biphasic medium
- Causes undulant fever, Malta fever
Brucella
specimen of choice for Bordetella pertussis
nasopharyngeal swab
- Cultured in BCYE
- isolated in air conditioning towers and heating systems
Legionella pneumophila
- animal bites and scratches
- Musty odor (odor of mushroom)
- non-motile
Pasteurella multocida
- Grows at 42℃
- seagull wings appearance
- darting motility
- Cultured in Skirrow’s medium and Campy’s agar
Campylobacter
- Agent of bioterrorism; causes anthrax (cutaneous, pulmonary, gastrointestinal)
- Medusa head colonies; bamboo pole or fishing rod appearance
Bacillus anthracis
Fried rice bacilli
Bacillus cereus
- Associated with Risus sardonicus
- produces tetanospasmin
- Drum stick appearance
Clostridium tetani
- Associated with clindamycin associated-pseudomembranous colitis
- Produces yellow colonies on Cycloserine Cefoxitin Fructose Agar
C. difficile
- Double zones of hemolysis
- (+) positive lecithinase
- Nagler plate
- (+) reverse camp
- Causes gas gangrene or myonecrosis
C. perfringens
- Produces botulism toxin which is associated with flaccid paralysis
- (+) Lipase
C. botulinum
Capnophilic and has gliding motility
Capnocytophaga
