Media Purpose

Question 1

Todd Hewitt W/ CNA

Answer 1

Enhance the growth of Group B Streptococcus. Used to find Group B Strep in pregnant women. Butt or Vag Swab

Question 2

Strep Selective Agar (Looks like Blood Agar)

Answer 2

Isolation of Group A Streptococcus

Question 3

CNA

Answer 3

Used for Group B Strep or Wounds
Isolates the growth of gram-positive bacteria, inhibits gram-negative bacteria.

Question 4

Chocolate Agar

Answer 4

Growth of a wide variety of organisms, especially growth of fastidious organisms. (Haemophilus/Neisseria)

Question 5

Modified Thayer-Martin (MTM) (Looks like chocolate agar)

Answer 5

Isolates Neisseria gonorrhoeae

Question 6

MacConkey Agar

Answer 6

Isolate and differentiate Gram negative bacilli, uses low concentration of bile salts to inhibit gpc

Question 7

MacConkey Agar w/ Sorbitol

Answer 7

Differentiate E. coli 0157: H7 from other gram-negative bacilli

Question 8

Hekton Agar

Answer 8

Differentiate Salmonella and Shigella form other gram-negative bacilli

Question 9

GN Broth

Answer 9

Enhance the growth of entero-hemorrhagic E. Coli and S. dysenteriae type 1 (Used to look for shigatoxins)

Question 10

Burkholderia Cepacia Agar

Answer 10

Isolate Burkholderia Cepacia, used in cystic fibrosis

Question 11

Mueller-Hinton Agar

Answer 11

E Test (Larger Disk) and Kirby-Bauer testing (Smaller Disk)

Question 12

Mycobiotic Slant
(Mycosel)

Answer 12

Recovery of Dimorphic fungi and Dermatophytes from specimens potentially contaminated with bacteria and saprophytic fungi

Question 13

SABHI Agar Slant

Answer 13

Recovery of pathogenic fungi

Question 14

Lowenstein-Jensen Slant

Answer 14

Isolation of Mycobacterium Species, esp. M. tuberculosis