media and gram staining Flashcards

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1
Q

what are the 2 types of media used for identifying microorganisms

A

selective and differential media

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2
Q

describe how selective media works

A

the presence of specific substances allows the growth of certain organisms over others, it selects the growth of specific prokaryotes

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3
Q

describe how differential media works

A

chemicals are used to produce visible changes in colonies that allow identification

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4
Q

what does the process of gram staining allow and how does it work

A

allows you to distinguish between the classes of gram +ve or -ve, according to the cell wall structure, using the retention of crystal violet/iodine complex by gram +ve bacteria

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5
Q

what are the limitations of gram staining

A

not all organisms stain well with gram staining, so other tests must be used for example the organism that causes TB

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