Media Flashcards
1
Q
What is the concentration of CO2 at 8000ft
A
Always 0.04%
2
Q
Concentration of glucose in media
A
Cm~ .1mM
BM~ 2.8
Glucose uptake and lactate production increase with bm
3
Q
What is the role of EDTA
A
A chelator of heavy metals
Used in Cleave media to prevent glycolosis, not blast media because needs glucose
4
Q
The embryo derives its centriolar apparatus and mitotic spindle from the spermatozoa, what could result from the use of a spermatozoa with a damaged centriole?
A
An embryo with severe cleavage arrest or irregular cleavage patterns
