Media

Question 1

○ nutrients are dissolved in water
○ bacterial growth is indicated by turbidity
○ grow small amounts of bacteria
○ provides longer viability of a sample

Answer 1

Broth (liquid)

Question 2

combination of a solidifying agent added
to the nutrients and water

Answer 2

Agar (solid)

Question 3

most common solidifying agent

Answer 3

Agarose

Question 4

used to observe and detect the motility of bacteria

Answer 4

Semi-solid

Question 5

used to enhance growth of a particular bacteria by providing specific nutrients required

Answer 5

Enrichment Media

Question 6

supports growth if most non-fastidious
organism

Answer 6

Nutritive Media

Question 7

contain inhibitory agents against a
specific bacteria, while allowing only a ‘selected’ bacteria to grow

Answer 7

Selective Media

Question 8

allow bacteria to exhibit metabolic / culture characteristic that can be distinguished

Answer 8

Differential Media

Question 9

Cultivation of fastidious organisms

Answer 9

Brain Heart Infusion (BHI)

Question 10

Cultivation of fastidious microorganisms such as Haemophilus spp., Brucella spp., and pathogenic Neisseria spp.

Answer 10

Chocolate Agar

Question 11

Selective isolation of gram-positive
cocci

Answer 11

Columbia CNA with Blood

Question 12

Selective (enrichment) liquid medium
for enteric pathogens (GN)

Answer 12

Gram Negative (GN) Broth

Question 13

Differential, selective medium for the
isolation and differentiation of Salmonella and
Shigella spp. from other gram-negative enteric
bacilli

Answer 13

Hektoen Enteric (HE) Agar

Question 14

indicator for the detection of H2S

Answer 14

ferric ammonium citrate

Question 15

routinely used for bacterial culture

Answer 15

Macconkey Agar, Chocolate Agar, Blood Agar Plate

Question 16

Isolation and differentiation of lactose fermenting and non-lactose fermenting enteric bacilli

Answer 16

Macconkey Agar

Question 17

Selective isolation of aerobic gram-positive cocci and bacilli

Answer 17

Phenylethyl Alcohol (PEA) Agar

Question 18

Cultivation of non-fastidious microorganism, determination of hemolytic reactions

Answer 18

Sheep Blood Agar (BA)

Question 19

one of the medias that show hemolytic
properties

Answer 19

Sheep Blood Agar (BA)

Question 20

Selective for N. gonorrhoeae and N. meningitidis

○ supports the growth of Francisella and Brucella spp.

Answer 20

Modified Thayer-Martin Agar (MTM)

Question 21

inhibit GN

Answer 21

Colistin

Question 22

inhibit GP

Answer 22

Vancomycin

Question 23

inhibit yeast

Answer 23

Nystatin

Question 24

inhibit Proteus spp.

Answer 24

Trimethoprim

Question 25

Enrichment broth or semi-solid,
supports growth of anaerobes, aerobes,
microaerophilic, and fastidious microorganisms

Answer 25

Thioglycollate Broth

Question 26

isolation and differentiation of
Salmonella (lactose fermenter) and Shigella spp.
from other gram negative enteric bacilli

Answer 26

Xylose - Lysine - Deoxycholate (XLD) Agar