Anaerobic Bacteria Flashcards
nonselective medium for isolation of anaerobes and facultative anaerobes
Anaerobic blood agar
selective and differential for Bacteroides fragilis group; presumptive identification
Bacteroides bile esculin (BBE) agar
selective for isolation of Prevotella and Bacteroides spp.
Laked Kanamycin-vancomycin (LKV)
selective for inhibition of enteric gram negative rods and swarming by some clostridia
Anaerobic phenylethyl alcohol (PEA) agar
nonselective for determination of lecithinase and lipase production by clostridia and fusobacteria
Egg yolk agar (EYA)
selective for Clostridium difficile
Cycloserine cefoxitin fructose sugar (CCFA)
nonselective for cultivation of anaerobic organisms with addition of glucose can be used for gas-liquid chromatography
Cooked meat (chopped meat) broth
nonselective for cultivation of anaerobic bacteria for gas-liquid chromatography
Peptone-yeast-extract-glucose broth (PYG)
nonselective for cultivation of anaerobes facultative anaerobes and aerobes
Thioglycollate broth
Add ________ for gram negative in first quadrant or ____ in second quadrant
Add kanomycin, vancomycin, or colistin disk for gram negative in first quadrant, or nitrate disk in second quadrant
Add ________ for gram positive cocci
Add nitrate and SPS
Add _________ for gram positive
Add nitrate for gram positive
Organism: Gram negative cocci
Legonella
Organism: Gram positive bacilli
Clostridium
may be placed on the second quadrant for subsequent determination of nitrate reduction
Nitrate Disk