Anaerobic Bacteria Flashcards

1
Q

nonselective medium for isolation of anaerobes and facultative anaerobes

A

Anaerobic blood agar

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2
Q

selective and differential for Bacteroides fragilis group; presumptive identification

A

Bacteroides bile esculin (BBE) agar

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3
Q

selective for isolation of Prevotella and Bacteroides spp.

A

Laked Kanamycin-vancomycin (LKV)

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3
Q

selective for inhibition of enteric gram negative rods and swarming by some clostridia

A

Anaerobic phenylethyl alcohol (PEA) agar

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4
Q

nonselective for determination of lecithinase and lipase production by clostridia and fusobacteria

A

Egg yolk agar (EYA)

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5
Q

selective for Clostridium difficile

A

Cycloserine cefoxitin fructose sugar (CCFA)

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6
Q

nonselective for cultivation of anaerobic organisms with addition of glucose can be used for gas-liquid chromatography

A

Cooked meat (chopped meat) broth

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7
Q

nonselective for cultivation of anaerobic bacteria for gas-liquid chromatography

A

Peptone-yeast-extract-glucose broth (PYG)

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8
Q

nonselective for cultivation of anaerobes facultative anaerobes and aerobes

A

Thioglycollate broth

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9
Q

Add ________ for gram negative in first quadrant or ____ in second quadrant

A

Add kanomycin, vancomycin, or colistin disk for gram negative in first quadrant, or nitrate disk in second quadrant

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10
Q

Add ________ for gram positive cocci

A

Add nitrate and SPS

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11
Q

Add _________ for gram positive

A

Add nitrate for gram positive

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12
Q

Organism: Gram negative cocci

A

Legonella

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13
Q

Organism: Gram positive bacilli

A

Clostridium

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14
Q

may be placed on the second quadrant for subsequent determination of nitrate reduction

A

Nitrate Disk

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15
Q

can be placed near the colistin disk for rapid presumptive identification of Peptostreptococcus anaerobius if gram positive cocci are seen in Gram staining

A

Sodium polyanethol sulfonate (SPS)

16
Q

may be added to the second quadrant
to detect bile inhibition if gram negative rods are seen on Gram staining

A

Bile disk