Med. App: Seven steps for Processing tissue for Light Microscopy

Question 1

Fixation

Answer 1

• Uses formalin or glutaraldehyde
• Preservation of normal tissue architecture after death
• Fixes coagulate or precipitate proteins (crosslinks prtns)
• Renders structures resistant to further processing

Question 2

Dehydration

Answer 2

• Uses a graded series of ethanol

- Removes water from tissue specimen and replaces it with alcohol

Question 3

Clearing

Answer 3

• Uses Xylene

- Prepares tissue for embedding medium

Question 4

Embedding

Answer 4

• Uses Paraffin

- Tissue is infiltrated with paraffin so that it is hard enough to section

Question 5

Sectioning

Answer 5

-Tissue is cut into thin sections with a microtome

Question 6

Mounting

Answer 6

-Cut parrafin section is placed on a glass microscope

Question 7

Staining

Answer 7

• Various chemicals

- Imparts contrast to tissue structures so that they may be distinguished upon microscopic observation

Question 8

Seven steps in Processing tissue for light microscopy

Answer 8

• FDCESMS
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• fixation, dehydration, clearing, embedding, sectioning, mounting, staining