Mechanism of enzyme action - the induced fit model Flashcards
1
Q
What needs to be drawn and labelled for the induced fit model
A
Three drawings:
1. An E shape represents the enzyme. Two circles joined by a bar represent the substrate. The active site is the 2 gaps in the ‘E’ shape.
- The 2 circles have been embedded in the E at the active sites and the active sites have changed shape to fit them, creating an enzyme-substrate complex
- Draw the ‘E’ shape representing the enzyme as it returns to its normal shape and two circles apart from it, not connected. These are the altered substrates that are the procucts
2
Q
List the 1st step in the induced fit model
A
- The substrate combines with the 3-D active site of the enzyme.
3
Q
List the 2nd step in the induced fit model
A
- The active site is induced or caused to change shape slightly by the substrate.
4
Q
List the 3rd step in the induced fit model
A
- The substrate and enzyme form an enzyme-substrate complex. The bonds in the substrate are altered so that the substrate changes into the product(s)
5
Q
List the 4th step in the induced fit model
A
- The products leave the active site.
6
Q
List the 5th step in the induced fit model
A
- The active site returns to its original shape and can now accept a new substrate molecule
7
Q
List the all 5 steps in the induced fit model
A
- The substrate combines with the 3-D active site of the enzyme.
- The active site is induced or caused to change shape slightly by the substrate.
- The substrate and enzyme form an enzyme-substrate complex. The bonds in the substrate are altered so that the substrate changes into the product(s).
- The products leave the active site.
- The active site returns to its original shape and can now accept a new substrate molecule
8
Q
At what speed do the 5 steps of the induced fit model happen?
A
They happen very quickly
9
Q
What can a small amount of enzyme process?
A
a large number of substrate molecules in a short space of time
