Mechanism of enzyme action: Lock + key, induced fit Flashcards
1
Q
What is a successful collision?
A
Where molecules involved need to collide successfully for resction to occur
2
Q
What is the activation energy?
A
Energy needed for reaction to begin
3
Q
What do enzymes do to activation energy?
A
They reduce it for reactions that they catalyse
Lock + key and induced fit are hypotheses explaining how this works
4
Q
Explain the lock + key hypothesis:
A
- Active site has complimentary shape to specific substrate
- Collide together + form enzyme-substrate complex
- Substrate reacts + forms enzyme-product complex, then products released
5
Q
What happens to the bonds within substrate when binding with active site (lock + key)?
A
R-groups within active site interact with substrate, forming temp bonds
This puts strain on bonds within substrate, also catalysing reaction
6
Q
Explain the induced-fit hypothesis:
A
- Active site changes shape slightly when substrate collides + binds to it (a confrontational change)
- Initial interaction between substrate + active site is weak
- Confrontational change of active site puts strain on substrate
- This may weaken bonds in substrate + lower activation energy for reaction
