Mechanism of action

Question 1

MOA of Tetracyclines?

Answer 1

Prevents protein synthesis/translation

Binds to the 30S ribosomal subunit at the Acceptor site; prevent the aminoacyl-tRNA molecules from binding to the A site.

Bacteriostatic

Tetracycline, Doxycycline, Minocycline, Tigecycline

Question 2

MOA of Fluoroquinolones?

Answer 2

inhibit bacterial DNA replication. They achieve this by targeting and inhibiting two enzymes essential for these processes: DNA gyrase and topoisomerase IV

Inhibit DNA strand separation and accumulation of unseparated DNA strand leads to DNA damage and cell death

Ciprofloxacin, Moxifloxacin, Levofloxacin

Question 3

MOA of Macrolides?

Answer 3

inhibit bacterial protein synthesis by binding to the bacterial 50S ribosomal subunit, specifically the 23S ribosomal RNA, and disrupting the translation process

prevent the ribosome from adding the next amino acid to the growing peptide chain, thus halting protein synthesis.

Bacteriostatic

Azithromycin, Clarithromycin, Erythromycin. (ACE)