Mechanism & Investigation of Diseases Flashcards
what does sensitivity tell us
tells us out of those who do have a disease, how many were tested positive
what does specificity tell us?
tells us out of those who do not have a disease, how many were correctly tested negative.
what does positive predictive value tell us
tells us out of those who were told they have the disease, how many actually do have
what does negative predictive value tell us
tells us out of those who were tested to not have the disease, how many truly do not
how to calculate positive likelihoood ratio?
sensitivity/1-specificity
how to calculate negative likelihood ratio?
1-sensitivity/specificity
how to calculate positive and negative predictive values?
PPV: (truly disease)/(tested positive)
NPV: (truly healthy)/(tested negative)
what is likelihood ratio used for?
to determine how good a test is at telling someone if they have the disease given a particular result.
what value of likelihood ratios are good?
if LR = 1 then test is useless
number should be much higher than 1.
what is a VUS in genomic study?
when someone has a variant of uncertain significance. means that they have a different genetic composition from ‘normal’, but we do not how that affects the person phenotypically
describe the components of the cellular innate defence
phagocytyes (macrophages) dendritic cells neutrophils NK cells other granulocytes like mast cells and, eosinophils and basophils
what do macrophages do
non-activated they help clear cellular debris
once activated they secrete cytokines that activate complement, endothelial cell adhesion molecules, and act as APC in lymph nodes
what kind of cells do NK cells target
tumour cells or virally infected cells
why is it that NK cells can kill tumour cells
because tumour cells lose MHC I expression
what surface molecule inhibit NK cell activity
MHC I
what can activated B cells become
plasma cells
memory b cells
APC
what is the first antibody that B cells secrete?
IgM
what is the process called when B cells increase their fit to the antigen
somatic hypermutation
what cells activate B cells?
T cells
what do CD4 and CD8 cells do?
CD4 express specific antigens on themselves, the circulate the body looking for APCs with matching antigen
once activate, they can activate B cells, or become CD8 cells, Treg cells or other Th cells
what cells express MHC II molecule?
immune cells like T cells, B cells, and APC
what cells express MHC I?
self cells
how is an immune response cleared?
after pathogen is cleared, cells stop becoming stimulated and naturally apoptose.
what are 2 mechanisms of cultivating self tolerance?
thymic central T cell selection
peripheral tolerance
what protein is key in central tolerance?
AIRE protein
what cell is key in peripheral tolerance?
Treg cells
what are 2 ways Treg cells can be made?
in the thymus or as progenitors of Cd4 t cells
what is the name of a primary loss of central self tolerance?
APECED
what is the name of the syndrome where peripheral tolerance is lost?
IPEX syndrome
2 other ways that self tolerance can fail other than central and peripheral self-tolerance loss
molecular mimicry
or chronic APC stimulation
describe type 1 2 3 4 hypersensitivity reactions and examples
type 1 - IgE mediated, mast cells, histamine release e.g. anaphylaxis
type 2 - IgG-IgM interaction on surface of target cell, tissue specific e.g. goodpastures, myesthaenia gravis,
type 3 - immune complex deposition e.g. SLE, RA, farmers lungs
type 4 - T cell mediated, organ specifice.g. Type 1 diabetes
what is the antigen targeted in goodpastures disease?
type 4 collagen
what is the pathophysiology of farmers lungs? what type HS?
type 3
patient breathes in allergen e.g. hay, triggers immune reaction, produces IgG antibodies against allergen whcih combine with allergen and forms immune complex which gets embedded in alveoli wall
chronic inflammation ensues, causing reduces lung function and clinical symptoms
acute management of anaphylactic shock?
Oxygen
adrenaline IM 0.5 mg in adults
antihistamine IV - chlorphenamine
corticosteroids IV
what must be measured after anaphylaxis?
serum tryptase
what are some primary immunodeficiencies
T cells, b cell
antibody, complement, neutrophil deficiencies
what type of infections are antibody, complement and neutrophils good at?
extracellular
what type of infections would someone be prone to if they are complement deficient?
pyogenic infections e.g. streptococcus and HIB
what type of infection would complement C5-9 deficiency lead to?
meningococcal
what does eculizumab inhibit?
complement
what does rituximab inhibit?
CD20 (B cell)
what treatment can be done for antibody, complement or neutrophil deficiencies
antibiotic, antifungal prophylaxis
Ig replacement
stem cell transplant
where is complement made?
liver
what type of infections would someone with T and B cell deficiencies be prone to?
viral, protozoal, intracellular infections, but also extracellular
how would someone with SCID present?
early on in life, around 6 months
with chronic LRTIs, fungal infections, PCP
what is the problem in autoimmune lymphoproliferative conditions?
failure to clear lymphocytes after infections, causing prolonged inflammatory state, causing autoimmune conditions
3 types of rejection in solid organ transplant
hyperacute
acute
chronic
mediators of each type of rejection in SOT?
hyperacute - preformed antibodies
acute - adaptive immune sytem T/B cells
chronic - antibodies, inflammatory system, fibrosis, scarring
e..g of hyperacute rejection?
preformed ABO antibodies
what is the most common cause of graft loss?
chronic rejection