measuring the hct

Question 1

measuring the hct

Answer 1

shake blood sample
transfer by capillary action blood to centrifuge tube to fill to 2cm from other end
seal end with wax
place in centriguge grooves - sealed end outwards
spin
blood separate into layer of cells at bottom and plasma at top
as tube is of uniform bore - length of red cell column compared to total length = Hct

Question 2

force from centrifuge

Answer 2

give 12000x force of gravity for 5 min

Question 3

getting blood in capillary tube

Answer 3

gradually flatten tube - to facilitate tube filling

Question 4

covering tube with wax

Answer 4

put finger over the tube
wipe end
dip twice into wax

Question 5

measuring the ratio

Answer 5

place tube into reader groove
line bottom line with blood putty interface
slide across so top line at line of plasma
use white lever so middle line is in line with the plasma cell interface - read off the R hand axis