Measles

Question 1

What is the classification of measles virus

Answer 1

Non segmented, paramyxovirus FAMILY, morbillivirus GENUS.

Question 2

How is measles infection start in the human body

Answer 2

Lower respiratory tract in the immune cells

Question 3

Draw the structure of a paramyxovirus ribonucleoprotein complex.

Answer 3

L polyemerase, Phosphorotein, nucleoproteins, and RNA

Question 4

What are the host cell receptors that measles can bind to?

Answer 4

CD46- on all nucleated cells
SLAM - on all T and B lymphocytes and APCs
Nectin 4 - epithelial cell receptor required for virus shedding in vivo

Question 5

What is the functional structure of the H protein in measles?

Answer 5

Tetramer

Question 6

What is the functional structure of the F protein in measles?

Answer 6

Trimer

Question 7

Draw the genome organisation of the measles virus

Answer 7

3’ - NP - P/C/V - M - F - H - L - 5’

Question 8

Describe the timeline of things that occur when measles infects a cell

Answer 8

1. transcription
2. translation of viral proteins
3. N proteins encapsidate newly synthesised RNA
4. antigenome is template for replication of new genome RNA

Question 9

what is the function of measles virus V and C protein

Answer 9

Inhibit interferon synthesis and signalling

Question 10

Compare measles and flu

Answer 10

SIMILAR-
- structure
-inhibition of IFN by NS1 in flu and V and C proteins in measles
DIFFERENT
- entry endosomal vs direct fusion, neutral Ph in measles vs acidified endosome in flu