HSV

Question 1

What is the size of the herpesvirus genome?

Answer 1

dsDNA 125kbp - 230kbp

Question 2

What family is HSV?
What subfamily is HSV?
What genus is HSV?

Answer 2

Family - herpesviridae
Subfamily - alphaherpesviridae
Genus - Simplexvirus (HSV type 1 and 2)
Varicellovirus - VZV

Question 3

What is the total length of the HSV1 genome

Answer 3

152kbp

Question 4

What are the attachment proteins for HSV1?

Answer 4

gB/gC.

Binds heparan sulphate proteoglycan on filopodia.

Question 5

What are the additional receptors required for entry of HSV1?

Answer 5

gD, gH, and gL

Question 6

What does gD bind to?

Answer 6

Nectins

Question 7

What are IE genes activated by?

Answer 7

VP16, tegument protein.

Question 8

How does VP16 activate IE genes?

Answer 8

Forms complex with HCF which targets it to the nucleus where it binds a target sequence

Question 9

What does binding of VP16/HCF to GArAT require?

Answer 9

Binding of host TF Oct1

Question 10

What is gene expression regulated by?

Answer 10

Immediate early protein ICP4

Question 11

What is ICP0?

Answer 11

Immediate Early protein

Essential for low moi infection to succeed.

Question 12

What does UL41 do?

Answer 12

Shuts off host protein synthesis by destabilising all mRNAs

Acts as an RNase, may target mRNA in polysomes through interaction with eIF4H

Question 13

How is UL41 inhibited in late infection?

Answer 13

Through binding to VP16

Question 14

Which proteins in HSV block host transcription and RNA splicing?

Answer 14

ICP22

ICP27

Question 15

What is UL30

Answer 15

DNA polymerase