MCP Flashcards
Coulomb’s Law
U=kq1q2/Dr
Where U is the energy needed to pull apart
Y axis of Scatchard Plot
% Occupancy/ [Ligand]
Complete Gibbs Equation
dG=dG’ + RTln(Products_initial)/(Reactants_initial)
Add the standard free energy to the RTln of the initial
How do you combine Keqs?
You MULTIPLY them.
What sign does the RT have when Gibbs is at equilibrium?
NEGATIVE
Gibbs free energy value at Equilibrium (Numerical)
Zero
How do you combine dG free energies?
You ADD them.
2nd Law of Thermo (Explanation and equation)
S=kbln(W)
Universe tends towards max disorder.
[Boltzmannln of possible arrangements]
Average H bond energy vs Average Covalent energy
H:5-30
Cov: 100+
X intercept of Scatchard plot
Maximum Hormone bound Bmax, or Number of binding sites
+H and -S
Nonspontaneous at all Temps
How can scatchard plot determine if there’s cooperativity or allostery?
deviations from linear slopes.
dG’, Gibbs equation at equilibrium
-RTln(Keq)
-H and +S
Spontaneous at ALL temperatures
Small Kd means higher or lower affinity?
Higher, because it means you need less ligand to get to the point where 50% is bound.
Dissocation constant kd
Inverse of Keq; so [A]*[B]/[AB]
Kd=Koff/Kon
Kd=Concentration when 50%bound to PL complex.
-H and -S
Spontaneous only at LOW temperatures. The Low Temp decreases contribution from S.
+H and +S
Spontaneous only at HIGH temperatures; High temp increases contribution from S
1st Law of Thermodynamics (Explanation and Equation)
Energy is conserved.
dU=q-w
Slope of scatchard plot
-Ka
Enthalpy (Explanation and equation)
The Heat in a system where energy is related to the pressure and the volume.
H=U+PV
