MCB

Question 1

State the two different regions in human genome

Answer 1

gene-dense ( 1 gene per 20kb) and gene-poor regions

Question 2

State the two human global genome clusters

Answer 2

alpha cluster (chromosome 16- 40kb) and beta cluster (chromosome 11-30kb)

Question 3

What is needed for the regulation of chromatin structure?

Answer 3

Acetylates, deacetylases and methylates- hypoacetylation causes recruitment of SIR2,SIR3 and SR4 proteins which cause condensation of nucleosomes and thus, closed chromatin

Question 4

How does ori works ?

Answer 4

• recruitment of proteins and DNA helices to form pre replicative complexes
• degradation of phosphorylated cdc6 by cyclin-dependent phosphorylation and phosphorylation of ORC occurs
• DNA helicase unwinds and enables the initiation of DNA replication

Question 5

How is ori essential(DNA replication during interphase) for cell division and cell cycle?

Answer 5

• ori initiates DNA replication ( during interphase) and makes sure daughter cells have same number of chromosomes after mitosis and cell division

Question 6

State crucial proteins required for 3’ polyadenylation.

Answer 6

• Cleavage and Polyadenylation specificity factor
• Cleavage Stimulation Factor
• Poly A polymerase
• Poly A binding protein