MBIO Lecture Test Flashcards
Who was Robert Hooke
He was the first to describe Microbes
Who was Antoni van Leuwenhoek
built microscopes 50-300x and saw microbes. Called them animacules
Who was Louis Pasteur
studied wine and beer production and discovered fermentation. He also developed Pasteurization.
Who disproved Spontaneous generation
Louis Pasteur
Who was Robert Koch
Studied anthrax and set the criteria needed for relating a specific microbe to a disease. (Think Koch’s Postulates
How to calculate No of Bacteria from a serial dilution.
No. of Colonies/ (Volume)(Dilution)
What is Bright field microscopy
Specimen visualized using contrast from specimen and surrounding.
What is Phase contrast
a phase ring helping to amplify the difference in the refractive index of a cell and its surrounding.
What is Dark-field Microscopy
A hollow cone of light hits the cell allowing only the light refracted by the cell to enter into the objective.
What is fluorescence microscopy
When we use the ability of a cell to fluorescence to allow us to view the cell.
What is Differential Interference Contrast
using polarized light that is sensitive to refraction allowing the cell and the cell structures to appear 3D
What is Confocal Scanning Laser Microscopy
the use of lasers in layers to allow us view the cell in 3D.
Can CSLM be used on live cells
Yes
What is Transmission electron Microscopy
the cell is cut very thinly and coated with a heavy metal and the lens swapped for magnets and light swapped with electrons.
What is Scanning Electron Microscopy
Specimen coated thinly with a heavy metal and electrons scattered on the specimen. Produces very accurate images.
