Math Ish Flashcards
Both C3 and C4 of glucose will be 14C labeled.
What would be the consequence(s) of a mutation in the glycolytic enzyme GAPDH that made hydrolysis (i.e.,cleaveage by water) of the thioester intermediate more likely than phosphorolysis (cleavage by inorganic phosphate)?Would you expect an organism with this mutation to be aerobic, anaerobic or both? Briefly explain.
This mutant would produce 3-PG from GAP, reducing the net yield of ATP fromglycolysis to 0 ATP/glucose because 1,3-BPG is not available for substrate-levelphosphorylation. Because there is no net gain of ATP via anaerobic metabolism of glucose, this organism must be an obligate aerobe.
– 59.1 kJ/mol.
Given the predicted shortage of crude oil, there has been much research done to engineer simple organisms toproduce important industrial feedstock chemicals from biomass sources (e.g., glucose derived from cellulose). On theback of the cover exam sheet is a pathway that has been engineered in bacteria for the industrial production of 3-hydroxypropionate from glucose. This was achieved by knocking out the TIM gene, and adding genes that encode fivenovel enzymes (Enz1 –Enz5) with activities similar to those you have studied from Chapter 13. The consumption orgeneration of ATP is shown explicitly for the novel steps of the pathway, however, the use of other cofactors/co-substrates (e.g., NAD+, NADPH, TPP, H2O, Pi, etc.) is not shown.
(a) Use your knowledge of metabolic chemistry toindicate enzyme types (i.e., “aldolase-like”; “dehydrogenase”, “kinase”, etc.)
(b) Draw the structures of the intermediates A – D.
(c) The production of 3-hydroxypropionate is greater under anaerobic conditions vs aerobic conditions. Explain. (Hint:Think of Louis Pasteur, and his study of glycolysis)
