Glycolysis Flashcards

1
Q

Draw R and P: Hexokinase reaction

A
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2
Q

T or F:

Liver hexokinase has lower Km to allow glucose export.

A

FALSE:

Liver hexokinase has higher Km to allow glucose export.

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3
Q

Draw R and P:

Phosphoglucose isomerase reaction

A
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4
Q

What does the “Phosphoglucose isomerase reaction” do?

A

1,2 carbonyl shift via “enediolate” intermediate

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5
Q

Draw the Intermidiate for the Phosphoglucose isomerase reaction

A
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6
Q

Draw the mechanism for the Phosphoglucose isomerase reaction

A
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7
Q

Draw R and P: Phosphofructokinase reaction

A
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8
Q

What is the ‘comitted step’ in glycolysis?

A

Phosphofructokinase reaction

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9
Q

Draw R and P: Aldolase reaction with labeled carbons

A
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10
Q

Explain the mechanism in the Aldolase RXN

A

Reaction mechanism for aldolase:

  • A protonated Schiff base intermediate (iminium ion) forms between thesubstrate and an active site lysine residue. This promotes the electrontransfer during the bond cleavage reaction.
  • An aspartate residue facilitates the reaction via GABC.
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11
Q

Draw the mechanism for the Aldolase RXN

A
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12
Q

Draw R and P:

A
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13
Q

What is the mechanism for the Triose phosphate isomerase RXN?

A
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14
Q

Draw R and P: GAPDH reaction

A
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15
Q

Draw the mechanism for the GAPDH rxn

A
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16
Q

Draw the protonation of NAD+ with structures

17
Q

Define:

substrate-level phosphorylation

A

Substrate-level phosphorylation is a metabolic reaction that results in the formation of ATP or GTP by the direct transfer of a phosphoryl (PO3) group to ADP or GDP from another phosphorylated compound.

18
Q

Draw R and P:

Phospoglycerate kinase reaction

19
Q

Draw R and P:

Phospoglycerate mutase reaction

20
Q

Draw the mechanism:

Phospoglycerate mutase reaction

21
Q

Explain the “detour” to make 2,3-BPG

22
Q

What explains these curves?

23
Q

Draw R and P:

Enolase reaction

24
Q

Draw the mechanism: Enolase reaction

25
Q

Draw R and P:

Pyruvate kinase reaction

26
Q

Draw in shorthand the entire energy investment phase with name abreviations for products, names of enzymes, and additional reactants/products.

27
Q

Draw in shorthand the entire energy generation phase with name abreviations for products, names of enzymes, and additional reactants/products.

28
Q

Describe fermentation

A

Fermentation reduces pyruvate and allows anaerobic glycolysis to continue.

29
Q

Which 3 steps of glycolsis are irreversible?

A

HK RXN
PFK RXN
PK RXN