Glycolysis Flashcards
Draw R and P: Hexokinase reaction
T or F:
Liver hexokinase has lower Km to allow glucose export.
FALSE:
Liver hexokinase has higher Km to allow glucose export.
Draw R and P:
Phosphoglucose isomerase reaction
What does the “Phosphoglucose isomerase reaction” do?
1,2 carbonyl shift via “enediolate” intermediate
Draw the Intermidiate for the Phosphoglucose isomerase reaction
Draw the mechanism for the Phosphoglucose isomerase reaction
Draw R and P: Phosphofructokinase reaction
What is the ‘comitted step’ in glycolysis?
Phosphofructokinase reaction
Draw R and P: Aldolase reaction with labeled carbons
Explain the mechanism in the Aldolase RXN
Reaction mechanism for aldolase:
- A protonated Schiff base intermediate (iminium ion) forms between thesubstrate and an active site lysine residue. This promotes the electrontransfer during the bond cleavage reaction.
- An aspartate residue facilitates the reaction via GABC.
Draw the mechanism for the Aldolase RXN
Draw R and P:
What is the mechanism for the Triose phosphate isomerase RXN?
Draw R and P: GAPDH reaction
Draw the mechanism for the GAPDH rxn
Draw the protonation of NAD+ with structures
Define:
substrate-level phosphorylation
Substrate-level phosphorylation is a metabolic reaction that results in the formation of ATP or GTP by the direct transfer of a phosphoryl (PO3) group to ADP or GDP from another phosphorylated compound.
Draw R and P:
Phospoglycerate kinase reaction
Draw R and P:
Phospoglycerate mutase reaction
Draw the mechanism:
Phospoglycerate mutase reaction
Explain the “detour” to make 2,3-BPG
What explains these curves?
Draw R and P:
Enolase reaction
Draw the mechanism: Enolase reaction
