Glycolysis Flashcards
Draw R and P: Hexokinase reaction

T or F:
Liver hexokinase has lower Km to allow glucose export.
FALSE:
Liver hexokinase has higher Km to allow glucose export.
Draw R and P:
Phosphoglucose isomerase reaction

What does the “Phosphoglucose isomerase reaction” do?
1,2 carbonyl shift via “enediolate” intermediate
Draw the Intermidiate for the Phosphoglucose isomerase reaction

Draw the mechanism for the Phosphoglucose isomerase reaction

Draw R and P: Phosphofructokinase reaction

What is the ‘comitted step’ in glycolysis?
Phosphofructokinase reaction
Draw R and P: Aldolase reaction with labeled carbons

Explain the mechanism in the Aldolase RXN
Reaction mechanism for aldolase:
- A protonated Schiff base intermediate (iminium ion) forms between thesubstrate and an active site lysine residue. This promotes the electrontransfer during the bond cleavage reaction.
- An aspartate residue facilitates the reaction via GABC.
Draw the mechanism for the Aldolase RXN

Draw R and P:

What is the mechanism for the Triose phosphate isomerase RXN?

Draw R and P: GAPDH reaction

Draw the mechanism for the GAPDH rxn

Draw the protonation of NAD+ with structures

Define:
substrate-level phosphorylation
Substrate-level phosphorylation is a metabolic reaction that results in the formation of ATP or GTP by the direct transfer of a phosphoryl (PO3) group to ADP or GDP from another phosphorylated compound.
Draw R and P:
Phospoglycerate kinase reaction

Draw R and P:
Phospoglycerate mutase reaction

Draw the mechanism:
Phospoglycerate mutase reaction

Explain the “detour” to make 2,3-BPG

What explains these curves?


Draw R and P:
Enolase reaction

Draw the mechanism: Enolase reaction

Draw R and P:
Pyruvate kinase reaction

Draw in shorthand the entire energy investment phase with name abreviations for products, names of enzymes, and additional reactants/products.

Draw in shorthand the entire energy generation phase with name abreviations for products, names of enzymes, and additional reactants/products.

Describe fermentation
Fermentation reduces pyruvate and allows anaerobic glycolysis to continue.

Which 3 steps of glycolsis are irreversible?
HK RXN
PFK RXN
PK RXN