Math For Lab

Question 1

In order to prepare 20L of 1X TAE buffer from 50X TAE stock solution, how much (in ml) of the 50X TAE buffer is diluted with water to prepare 20L of 1X TAE?

Answer 1

400ml

Question 2

In this mixture, the ratio between components A:B:C:D is 8:5:3:1 respectively. In order to create 170ml of this mixture, how much of liquid C is needed?

Give answer in ml.

Answer 2

30 ml

Question 3

How much EDTA in grams is needed to make 250ml of 0.5M EDTA?

Assume that EDTA has a molecular mass of 292 gram per mole.

Answer 3

36.5 gm

Question 4

How much H2SO4 in grams is needed to create 2.5L of a 0.8M solution?

Assume that the molecular mass is H is 1g, S is 32g, and O is 16g.

Answer 4

196g

Question 5

How much NaCl in grams is needed to create 250ml of a 0.4M solution?

Assume that the molecular mass of Na is 23g and Cl is 35.5g.

Answer 5

5.85g

Question 6

In order to create a 50ml stock solution of 10mg/ml ethidium bromide, how much ethidium bromide (in mg) is needed?

Answer 6

500mg

Question 7

In order to prepare 50ml of 0.8% agarose, how much agarose (in mg) is needed?

Answer 7

400mg