Mass Spectrometry Flashcards
What steps may be required to prepare samples for MS?
Sample prep:
homogenization, dry/weigh, extraction (LLE, LPME, SPE…), purification/clean up, preconcentration, chem. modification (if needed)
separation:
LC, GC, IC, CE, FFF…
*or can be direct analysis
How is exact atomic mass different from the molecular mass?
molecular mass is AVERAGE from relative abundance of various natural isotopes
each natural isotope for a element will have a different exact mass
What are the most abundant forms of H, C, N, O, and S?
1H, 12C, 14N, 16O, 32S
What is the y and x axis in a mass spectrum?
y axis: relative intensity
x axis: m/z
What is m/z?
mass to charge ratio
divide mass number of ion by charge number
What environment is necessary for mass spectrometry?
vacuum (10-5 to 10-8 torr)
What are the general parts of a mass spectrometer? (6)
inlet system (connect to GC, LC….)
vacuum system
ion source (produce gas-phase ions)
mass analyzer (ions separated according to m/z)
detector (collect/convert specific ions into electric signal w/ ion transducer)
computer/signal processor (control + collect/analyze data)
The MS is connected to the LC or GC or CE by the ___ ____
inlet system
Where are the ions separated in the MS?
Mass analyzer (separate according to m/z)
The detector will _____ ions with a certain ____, and convert into an electric signal using a ___ _____
collect
m/z
ion transducer
What are common ion sources for mass spectrometry? (5)
gas phase:
- electron impact (EI)
- Chemical ionization (CI)
Desorption:
- Fast atom bombardment (FAB)
- matrix assisted laser desorption/ionization (MALDI)
- Electrospray ionization (ESI)
What is the method of ionization for EI? what type of spectra does it produce?
energetic electrons
fragmentation patterns
What is the method of ionization for CI? what type of spectra does it produce?
reagent gaseous ions
proton adducts, few fragments
What is the method of ionization for FAB? what type of spectra does it produce?
energetic atom beam
molecular ions and fragments
What ionization type produces proton adducts?
chemical ionization
What method of ionization uses high energy photons?
MALDI
what type of spectra does MALDI produce?
molecular ions, multiply charged ions
What ionization methods can produce multiply charged ions?
MALDI
ESI