Marta Irla - Synthetic Biology Flashcards
The Figure shows the response of a B. clausii-derived metE riboswitch (reflected by the level of gene expression for a β-galactosidase reporter gene fused to the 5′ UTR of the B. clausii metE mRNA) to supplementation of growth media with methionine (Met) and adenosylcobalamin (AdoCbl).
Knowing that any decrease in the gene expression can be interpreted as activation of the riboswitch, say what type of logic gate this riboswitch represents, include 2-3 sentences of explanation.
Explain how the knowledge about regulation of gene expression can be applied for design of synthetic pathways. Choose one example of functional element for regulation of gene expression and explain which type of logic gate it represents.
What are the advantages of using synthetic microbial consortia over the monocultures (at least 3)? What approaches can be used in design of synthetic microbial consortia (at least 3), briefly explain them and give at least one example of biotechnological applications where they have been applied (one example for each approach in design listed).
What are the preferred properties of target organism used for top-down approach in creation of minimal cell. Explain the process of creating a minimal cell in a top-down approach.
Explain the process of designing a minimal cell bottom-up approach. What are the essential features and processes that should be considered?
