manipulating genomes Flashcards
diff between somatic and germ like therapy
somatic can target specific tissue
germ line cannot target specific tissue
order of dna sequencing
extract sample
cut dna into sections of varying length
amplify dna
sequence shirt sections of dna
place sections in order of matching over lapping regions
things which have led to increased speed in dna sequencing
pyrosequmcing
high thourough put sequencing
whole human genetic genome
how does dna sequencing allow sequence if amino acids in polypeptide to be predicting
by looking at the sequence of bases and it tells u the codon which is formed and therefore the amino acid
how can dna sequencing and bioinformatics be used to increase effectiveness of vaccine programmes
dna sequencing - be able y predict strains and produce vaccine w correct antigen
bioinformatics - can analyse and store large ants of data to identify the source of the outbreak by making comparisons
how can bioinformatics be used to identify whether a new allele causes genetic disease
base sequence of normal allele is help in database
computational biology allows analysis of rapid comparison
why are booster vaccines needed
memory cells may have reduced in number
why are only non coding sections used when profiling humans
overall genome is very similar
the introns contain tan form repeats which vary in length for each individual this can be used to compare length size of tandem repeats of different inidivis
why may PCR not occur effectively
lack of primers
too high temp causes damage
lack of nucleotides
why is taq polymerase used and not normal one
the enzymes don’t nature at 95°
and so ocr can be repeated again and again without stopping
diff between tlc and electrophoresis
adsorbance vs charge
electricity vs solvent
dyes vs fluorescent tags
how can pcr be used to compare growth rates in healthy vs cancerous tissue
extract dna from both types of tissue
compare rate of dna aplification after certain number of cycles
why are there less genetically engineering concerns for bacteria
less animal rights to consider
adv of us isn’t GM rather than spraying
GM is more effective. and we can be sure it is working we can’t be sure for the other
