Manipulacion De Genes Flashcards

0
Q

Endonucleas

A

Son enzimas de restriccion.

Cortan los enlaces fosfodiester del DNA

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1
Q

Nucleasas de restriccion

A

Rompen el DNA

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2
Q

Endonucleasas

A

Reconoces secuencias palindromicas

Son extraidas de bacterias

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3
Q

Enzimas de restriccion pueden producir 2 extremos:

A
  1. Cohesivos-cortan escalonados

2. Ramos/abruptos- cortan solo 1 punto

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4
Q

Dna recombinante

A

Moleculas de dna producidas mediante la union de 2 o mas fragmentos de DNA

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5
Q

Gel de agarosa

A

Extraido de algas

Corta de 200-500pb

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6
Q

Factores que afectan la velocidad de migracion

A

Tamaño de fragmentos de DNA
Concentracion de agarosa
Voltaje aplicado
Composicion del buffer

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