Malaria stuff Flashcards

1
Q

Malaria is a parasitic infection caused by the introduction of the protozoan organisms into the blood by the bite of a ……

A

female anopheles mosquito

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2
Q

Life cycle of plasmodium
Female Anopheles mosquito bites human.
_______ injected into human when bitten

A

Sporozoites

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3
Q

Life cycle of plasmodium (malaria parasite)
Sporozoites enter parenchymal cells of liver to become ________.
(a) Development in liver: _______ cycle.
(b) Further development in the liver after RBC invasion: ______ cycle.

A

schizonts

(a) Pre-erythrocytic
(b) Exoerythrocytic

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4
Q

Life cycle of plasmodium (malaria parasite)
Inside parenchymal cells, Schizonts rupture, releasing ________, which then burst out of liver cells to invade RBCs.
(a) Merozoites develop to form immature (ring stage) ______.
(b) Become actively _____.

A

merozoites

(a) trophozoites
(b) amoeboid

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5
Q

When does this happen

Beginning of sexual reproduction - production of zygote

A

When the Gametocytes are ingested by mosquito

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6
Q

All stages of asexual cycle can be demonstrated in a ________ with the exception of ________

A

peripheral blood smear

P. falciparum

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7
Q

types of malaria found in humans
It is observed that the fever cycle corresponds to
the _______ cycle for each of the malaria species

A

Erythrocytic Schizogony

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8
Q

What type of malaria

(a) Erythrocytic cycle (Malignant Tertian): Fever every 48 hours or every 2nd day.
(b) Most Fatal: Likely to cause intravascular hemolysis (destruction of the RBCs).
1) Fever is prolonged and intensified.

A

Plasmodium Falciparum

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9
Q

What type of malaria

(a) Erythrocytic cycle (Benign Tertian): Fever every 48 hours or every 2nd day.
(b) Most common.

A

Plasmodium Vivax

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10
Q

What type of malaria

(a) Erythrocytic cycle (Benign Tertian): Fever every 48 hours or every 2nd day.

A

Plasmodium Ovale

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11
Q
What type of malaria
Erythrocytic cycle (Benign Quartan): Fever every 72 hours or every 3rd day.
A

Plasmodium Malariae

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12
Q

Specimen collection and storage
Capillary Collection
With a lancet, puncture the skin and collect the blood directly into the ________ (included in the test kit).

A

EDTA capillary tube

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13
Q

Specimen collection and storage
Capillary Collection
Fill the entire capillary tube with blood and test _____

A

immediately

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14
Q

How many types of malaria is found in humans?

A

4

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15
Q

Specimen collection and storage: Venous blood

Collect blood into an _____

A

EDTA tube (purple top)

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16
Q

True/false
Specimen collection and storage: Venous blood
Test whole blood as soon as possible after collection

A

TRUE

17
Q

Specimen collection and storage: Venous blood

The blood sample may be stored for up to _____ days at ________ if cannot be tested immediately

A

three

2°C to 30°C (36-86°F)

18
Q

Specimen collection and storage: Venous blood

IF blood sample is refrigerated, allow blood sample to reach what temperature prior to performing test?

A

room

temperature (15-30°C)

19
Q

Rapid Malaria test

What is Tris buffer containing detergent and sodium azide

A

Reagent A

20
Q

Testing procedure: Priming the Sample (purple) pad

For venipuncture method: Collect ___ microliters of blood sample with a calibrated pipette and place on the sample pad.

A

15 microliters

21
Q

Testing procedure: Priming the reagent (white) pad

Place ___ drops of Reagent A onto the reagent pad. Allow the first drop to ______ before placing the next drop.

A

2

absorb

22
Q

Testing procedure: Priming the reagent (white) pad

If the mixture does not run through the whole test strip in a minute, then….

A

an additional drop of reagent a must be applied

23
Q

Testing procedure: Priming the reagent (white) pad

Read test result through the viewing window at _______. Results are ______ if read after 15 minutes.

A

15 minutes

invalid

24
Q

Result interpretation

The “C” Control line must appear in order for the test to be _____

A

valid

25
Q

Result interpretation

T1

A

Positive for Plasmodium Falciparum

26
Q

Result interpretation
What result will show for: Positive for Plasmodium Vivax, Malariae, or Ovale. May indicatemixed infection of the three malaria parasites.

A

T2

27
Q

Result interpretation

What will show for Positive for Plasmodium Falciparum and other types of malaria in a mixed infection

A

T1 and T2

28
Q

Result interpretation

Only the C line

A

Indicates the test is negative

29
Q

The test is invalid if the _____ does not appear.

A

Control “C” line

30
Q

TRue/FAlse

Can be used to monitor treatment progress

A

FALSE

CANNOT

31
Q

False positives results in patients with what conditions?

A

(a) Rheumatoid arthritis
(b) Chronic viral infections - Hepatitis C
(c) Patient with other blood parasite – Babesia

32
Q

Collect specimen immediately after onset of _____ for best results

A

fever spike

33
Q

Send unstained smears to the _______ as soon as possible for identification and confirmation.

A

Navy Environmental and Preventive Medicine

Unit (NEPMU)

34
Q

Smears should be done frequently but no more than ________

A

hourly

35
Q

Samples must be obtained when patient is _____

A

Febrile

36
Q

True/false

The test will only detect the antigens

A

True

37
Q

Rapid Malaria kit Limitations

Must be confirmed with a thick and thin smear for confirmation, sent to

A

NEPMU

38
Q

All stages of asexual cycle can be demonstrated in a peripheral blood smear with
the exception of

A

P. falciparum