magnification Flashcards

1
Q

what is resolution

A

the ability of an optical instrument to produce an image that shows fine detail and is able for us to distinguish between two different points

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

what is magnification

A

the ability of an object to produce an image bigger than the original objects

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

features of a light/optical microscope

A

poor resolution and magnification due to longer wavelengths of light .

cant see smaller organelles

cheap, portable

easy to use

in colour

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

features of an electron microscope

A

shorter wavelength so higher resolution and higher magnification

uses beam of fast travelling electrons

sample must be dead or in vacumm so electrons arent absorbed by air or any interference by other molecules

has to be stained as in black and white

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

features of transmission electron microscope

A

extremely thin specimen is stained and placed in vacumm

electron gun produces beam of electrons that pass through specimen

some parts of specimen absorb more electrons making them denser

high resolution and magnification

2d cell image in black and white

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

scanning electron microscopes

A

specimen dosent need so be thin as electrons are beamed onto the surface which then causes secondary electrons to bounce and scatter and then focus onto the screen

3d image produced

lower resolution and magnification than tem

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

image size = ?

A

real size times magnification

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

why cant optical microscopes produce bigger images

A

the wavelength of optical microscopes are the same of visible light (400-700nm) which is low and for this reason it cant produce an image smaller than the wavelnght of light

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

what is the mag and res of a light microscope

A

mag 1500x res 0.2 micrometres

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

mag and res of tem microscope

A

mag more than 1,000,000x res 0.0005micrometres

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

mag and res sem microscope

A

mag under 1,000,000x res 0.003-0.005

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

animal cell organelles that can be seen by light microscope

A

cell surface membrane, cytoplasm, nuceus , mitochondria, centriole , cytoplasmic granules

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

plant cell organelles that can be seen by a light microscope

A

cell wall, cell surface membrane,nucleus,cytoplasm,vacuole , tonoplast, mitochondria, starch grain, chloroplast

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

animal cell organelles that can be seen by electron microscope

A

endoplasmic reticulum, nucleoulus,lysosome ,ribosome,nuclear envelopewith pores, golgi apparatus

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

plant cell organelles that can be seen with electron microscope

A

er,plasmoderma,nucleoulus,ribosome,
nuclear envelope w/pores,golgi apparatus

How well did you know this?
1
Not at all
2
3
4
5
Perfectly