magnification

Question 1

what is resolution

Answer 1

the ability of an optical instrument to produce an image that shows fine detail and is able for us to distinguish between two different points

Question 2

what is magnification

Answer 2

the ability of an object to produce an image bigger than the original objects

Question 3

features of a light/optical microscope

Answer 3

poor resolution and magnification due to longer wavelengths of light .

cant see smaller organelles

cheap, portable

easy to use

in colour

Question 4

features of an electron microscope

Answer 4

shorter wavelength so higher resolution and higher magnification

uses beam of fast travelling electrons

sample must be dead or in vacumm so electrons arent absorbed by air or any interference by other molecules

has to be stained as in black and white

Question 5

features of transmission electron microscope

Answer 5

extremely thin specimen is stained and placed in vacumm

electron gun produces beam of electrons that pass through specimen

some parts of specimen absorb more electrons making them denser

high resolution and magnification

2d cell image in black and white

Question 6

scanning electron microscopes

Answer 6

specimen dosent need so be thin as electrons are beamed onto the surface which then causes secondary electrons to bounce and scatter and then focus onto the screen

3d image produced

lower resolution and magnification than tem

Question 7

image size = ?

Answer 7

real size times magnification

Question 8

why cant optical microscopes produce bigger images

Answer 8

the wavelength of optical microscopes are the same of visible light (400-700nm) which is low and for this reason it cant produce an image smaller than the wavelnght of light

Question 9

what is the mag and res of a light microscope

Answer 9

mag 1500x res 0.2 micrometres

Question 10

mag and res of tem microscope

Answer 10

mag more than 1,000,000x res 0.0005micrometres

Question 11

mag and res sem microscope

Answer 11

mag under 1,000,000x res 0.003-0.005

Question 12

animal cell organelles that can be seen by light microscope

Answer 12

cell surface membrane, cytoplasm, nuceus , mitochondria, centriole , cytoplasmic granules

Question 13

plant cell organelles that can be seen by a light microscope

Answer 13

cell wall, cell surface membrane,nucleus,cytoplasm,vacuole , tonoplast, mitochondria, starch grain, chloroplast

Question 14

animal cell organelles that can be seen by electron microscope

Answer 14

endoplasmic reticulum, nucleoulus,lysosome ,ribosome,nuclear envelopewith pores, golgi apparatus

Question 15

plant cell organelles that can be seen with electron microscope

Answer 15

er,plasmoderma,nucleoulus,ribosome,
nuclear envelope w/pores,golgi apparatus