MAGNESIUM

Question 1

Magnesium

reagent

Answer 1

BLANK STANDARD PATIENT
1000 uL or 1 mL

Question 2

Magnesium

Serum/plasma

Answer 2

10 uL

Question 3

Magnesium

Distilled H2O

Answer 3

10 uL

Question 4

Magnesium

Standard

Answer 4

10 uL

Question 5

Magnesium

Mix and incubate

Answer 5

37C for 2 minutes

Question 6

Magnesium

Absorbance

Answer 6

546 nm

Question 7

Magnesium

CALCULATION

Question 8

Magnesium

Adult

Answer 8

1.30 – 2.10 mEq/L (0.65 – 1.05 mmol/L)

Question 9

Magnesium

Newborn (2-4 days)

Answer 9

1.20 – 1.80 mEq/L (0.60 – 0.90 mmol/L)

Question 10

Magnesium

5 months – 6 yrs.

Answer 10

1.42 – 1.88 mEq/L (0.71 – 0.94 mmol/L)

Question 11

Magnesium

6 – 12 years

Answer 11

1.38 – 1.74 mEq/L (0.69 – 0.87 mmol/L)

Question 12

Magnesium

12 – 20 years

Answer 12

1.35 – 1.77 mEq/L (0.67 – 0.88 mmol/L)

Question 13

Magnesium

Method

Answer 13

Dye-binding Method (Xylidyl blue)

Question 14

Magnesium

[?] combines with [?] at alkaline pH to form a [?], the absorbance of which is measured at [?]. Interference from other cations are avoided by specific [?]

Answer 14

Xylidyl blue

magnesium

purple complex

546 nm

chelating agents.

Question 15

Magnesium

Avoid [?] as anticoagulant

Answer 15

chelating agent

Question 16

Magnesium

Reagent Composition:

Answer 16

Xylidyl blue (Rgt 1 - blue cap)
NaCl
EGTA
Triethanolamine
Good’s buffer (pH 11.0) - maintains ph at 11
Surfactant Preservative

Question 17

Magnesium

Serum or plasma samples are stable at

Answer 17

2 - 8C for one week.

Question 18

Magnesium

[?] (preferred) or heparinized plasma

Answer 18

Serum

Question 19

Magnesium

Do NOT use [?] as anticoagulant.

Answer 19

citrate, oxalate and EDTA

Question 20

Magnesium

The specimen should be drawn without [?] if possible.

Answer 20

venous stasis

Question 21

Magnesium

Do not use samples from patients in therapy with[?].

Answer 21

EDTA

Question 22

Magnesium

Remove serum from [?].

Answer 22

clot without delay