CHLORIDE

Question 1

Chloride (Cl- ) Reagent

Answer 1

BLANK STANDARD PATIENT
1000 uL or 1 mL

Question 2

Chloride (Cl- ) Serum

Answer 2

5 uL

Question 3

Chloride (Cl- ) Water

Answer 3

5 uL

Question 4

Chloride (Cl- ) Standard

Answer 4

5 uL

Question 5

Chloride (Cl- ) Mix and incubate

Answer 5

37C for 5 minutes

Question 6

Chloride (Cl- ) Absorbance

Answer 6

480 nm

Question 7

Chloride (Cl- ) CALCULATION

Answer 7

100 mEqL

Question 8

Chloride (Cl- ) EXPECTED VALUES

Answer 8

98 – 110 mEq/L

Question 9

Chloride (Cl- ) Method

Answer 9

Colorimetric Method Based on the Modification of Skeggs and Hochestrasser

Question 10

Chloride ions form a [?] with [?] and will displace [?] from [?].

Answer 10

soluble, non-ionized compound

mercuric ions

thiocyanate ions

non-ionized mercuric thiocyanate

Question 11

The released[?] react will [?] to form a color complex that absorb light at [?]. The intensity of the color produced is [?] to the chloride concentration.

Answer 11

thiocyanate ions

ferric ions

480 nm

directly proportional

Question 12

Chloride (Cl- ) Ferrothiocyanate

Answer 12

(red colored)

Question 13

Chloride (Cl- ) More red =

Answer 13

higher Cl conc

Question 14

1. Chloride reagent(Active ingredients):

Answer 14

Mercuric Nitrate 0.058 mM
Mercuric thiocyanate 1.75 mM
Mercuric chloride 0.74 mM
Ferric Nitrate 22.3 mM

Question 15

Non-reactive ingredients and stabilizers in

Answer 15

dilute acid and methanol

Question 16

Precautions: [?] is POISONOUS. It contains mercury and [?]. Maybe harmful or fatal if swallowed. DO NOT PIPET BY MOUTH. Call physician if taken internally.

Answer 16

Chloride reagent

methanol

Question 17

2. Chloride calibrator:

Answer 17

Sodium chloride 100 mEq/L

Question 18

Chloride (Cl- )

1. Use serum that has been separated from the [?] soon after drawing.

Answer 18

clot

Question 19

Chloride (Cl- )

2. [?] should not be used as it may create falsely decreased values.

Answer 19

Grossly hemolyzed serum

Question 20

Chloride (Cl- )

3. Avoid contamination of blood with [?].

Answer 20

tissue fluid

Question 21

Chloride (Cl- )

4. Store serum in [?].

Answer 21

tightly stoppered tubes

Question 22

5. Chloride is stable in serum for [?] and for [?] when stored tightly capped.

Answer 22

1 day at room temperature

3 moths frozen