CHLORIDE Flashcards
Chloride (Cl- ) Reagent
BLANK STANDARD PATIENT
1000 uL or 1 mL
Chloride (Cl- ) Serum
5 uL
Chloride (Cl- ) Water
5 uL
Chloride (Cl- ) Standard
5 uL
Chloride (Cl- ) Mix and incubate
37C for 5 minutes
Chloride (Cl- ) Absorbance
480 nm
Chloride (Cl- ) CALCULATION
100 mEqL
Chloride (Cl- ) EXPECTED VALUES
98 – 110 mEq/L
Chloride (Cl- ) Method
Colorimetric Method Based on the Modification of Skeggs and Hochestrasser
Chloride ions form a [?] with [?] and will displace [?] from [?].
soluble, non-ionized compound
mercuric ions
thiocyanate ions
non-ionized mercuric thiocyanate
The released[?] react will [?] to form a color complex that absorb light at [?]. The intensity of the color produced is [?] to the chloride concentration.
thiocyanate ions
ferric ions
480 nm
directly proportional
Chloride (Cl- ) Ferrothiocyanate
(red colored)
Chloride (Cl- ) More red =
higher Cl conc
- Chloride reagent(Active ingredients):
Mercuric Nitrate 0.058 mM
Mercuric thiocyanate 1.75 mM
Mercuric chloride 0.74 mM
Ferric Nitrate 22.3 mM
Non-reactive ingredients and stabilizers in
dilute acid and methanol
Precautions: [?] is POISONOUS. It contains mercury and [?]. Maybe harmful or fatal if swallowed. DO NOT PIPET BY MOUTH. Call physician if taken internally.
Chloride reagent
methanol
- Chloride calibrator:
Sodium chloride 100 mEq/L
Chloride (Cl- )
- Use serum that has been separated from the [?] soon after drawing.
clot
Chloride (Cl- )
- [?] should not be used as it may create falsely decreased values.
Grossly hemolyzed serum
Chloride (Cl- )
- Avoid contamination of blood with [?].
tissue fluid
Chloride (Cl- )
- Store serum in [?].
tightly stoppered tubes
- Chloride is stable in serum for [?] and for [?] when stored tightly capped.
1 day at room temperature
3 moths frozen
