Macrophages Flashcards
What can you add to moncytes for Dc differentiation?
GM-CSF and IL-4
What is a very good marker for macrophages in the mouse?
F4 80.
Where can you find F4-80 macrohpages generally?
Stromal macrophages in the bone marrow,.
Lie around endothelium are sinusoidal macrophages (blood).
Lie under endotheilum, are perivascular macrophages.on tissue side.
Or tissue resident.
lie under epithelium or over and free of epithelium.
What role can stromal marcohpags play in RBC development?
During their dev.RBC associate with macrophage stromal cells and lose nucleus,
Nucleus engulfed.
What do other myeloid cells (not blood cells) use to attach to stromal macrophages?
sialo adhenin (CD169).
Where in the spleen is there a population of F4 80 macrophages?
In the white pulp they don’t express F4 80 (but do in red pulp for clearance of senescent RBCs.)
Where in spleen is there a CD169 (sialoadhesin population)
In the marginal zone. Shows microhetergeneity.
Where is there hetergenoity in the microglia?
start with stubby processes that develop.
Longer in grey matter than white matter.
Develop with age too.
What function do microglial play in brain development? What is this process dependent on?
Importat for motor funtion and refinement of synapses.
Process dependent on complement. (C1q and C3).
What is the difference between Csf-1 and Csf-2?
M-CSF (csf-1) and Gm-CSF= csf2.
What does transcriptome analysis of tissue macrophage pouplation show?
core genes + tissue specific genes.
Also microhetergeneity.
What connnexin links macrophages to the cardiomyotcytes?
connexin 43.
What do macrophages around AV node do?
MOdulate electrical activity of cardiomyoctyes, if KO then you get bradycardia.
What other function do macrophages have in heart?
Phagoctyose exophers from heart cells that contain predominantly dysfunctional mitochondria.
How do macrophages detect exophers?
with MERTk receptor for PS on exophers.
What does inflmmation do to the exphoer and autphagsomal pathway?
inihbits it when exophers not degraded.
What kind of cellular interactions do macrophages have?
lymphocytes (T and B cells), tumours cells (MDSC) and apoptotic cells, and themselves (fusion).
What pathogens can they recongise?
viruses, extra and intra cellular bacteria, funig.
After priming and activation (by TLR stimuli) what is the next step?
resolution- TGF-B IL-10 and lipoxines from the arachadnoic pathway.
Are sinuisoidal kupffer cells F480 positive or negative?
They are negative.
What is CD68 a marker of?
tissue macorphages and monocyte derived macrophages.
What is the pH drop in the pahagosome mediated by?
vacuolar ATPase, then lysoszyme pH contineus to drop.
3 ways pathogens can interefer with the membranes of phagocsomes to persist?
Viruses can fuse with the membrane and escape.
Other types of membrane disruption for escape.
Blocknig fusion of phagosomewith lysosomes and organelles.
What signalling molecule is involed in dectin signalling?
syk, synegises with TLRs to enhace cytokine and chemokine production.
Two ways of respiratory bursts killing?
Production of ROS that can directly damage pathogen.
Or fusion with other vacuoles containgin myeloid peroxidase and catalase containing peroxisomes to produce toxic oxidants from H2020 (HOCL?)
what are giant cells in Tb called?
Langhans cells
What pro live signal does SIRPa bind to?
CD-47 is an anti-apoptotic signal.
