Mac 9: P Flashcards

1
Q

Complete hydrolysis of nucleotides yields a mixture of what components? (Hint: 3 components)

A
  • Pentose Sugar
  • Phosphoric Acid
  • Base
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

The sugar in RNA

  • Deoxyribose
  • Ribose
A

-Ribose

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

After doing the experiment for Preparation of RNA from yeast and Preparation of Nucleoproteins, where do you dispose the chemicals used?

  • Corrosive waste - Inorganic/acid mixture Waste Disposal
  • Organic Non-halogenated Waste Disposal
A

-Organic Non-halogenated Waste Disposal

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

It is a conjugated protein consisting of a protein linked to a nucleic acid, either DNA or RNA.

  • Nucleotide
  • Nucleus
  • Nucleoprotein
  • Nucleolus
A

-Nucleoprotein

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

True or False

Freshly extracted yeast RNA will produce a brick red precipitate in Benedict’s test.

A

-False (No Brick Red Precipitate)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

True or False

Complete hydrolysis of RNA gives nucleotides.

A

-False (Gives Phosphoric Acid, Ribose & Bases)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

True or False

The presence of purine bases is indicated by the yellow precipitate when ammonium molybdate is added to the sample.

A

-False (White Precipitate)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

True or False
This type of nucleic acid is single stranded and an intermediate in the expression of genetic information and other diverse roles

  • RNA
  • DNA
  • Nucleus
  • Genes
A

-RNA

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

True or False

Hydrolyzed RNA will give a yellow solution in Bial’s Test.

A

-False (Hydrolyzed Pyrophosphate)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

The pyrimidine base found in DNA but not in RNA

  • Thymine
  • Uracil
  • Adenine
  • Cytosine
A

-Thymine

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

This reagent is used to wash the yeast RNA to remove potential interference in the biochemical test

  • Ethanol
  • HCl
  • NaOH
  • Sodium Hydroxide
A

-Ethanol

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

The sugar in DNA

  • Deoxyribose
  • Ribose
A

-Deoxyribose

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

What indicates a positive result in the Bial’s test

  • Blue Solution
  • Green Solution
  • Blue-Green Solution
  • Pink Solution
A

-Blue-Green Solution

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

The pyrimidine base found in RNA but not in DNA

  • Thymine
  • Uracil
  • Adenine
  • Cytosine
A

-Uracil

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

True or False

Nucleoproteins are proteins that are linked to nucleic acid?

A

-True

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Why is 10% NaOH important in preparing yeast RNA?

  • This extracts the nucleic acid and water-soluble proteins and inactivates the nucleases.
  • It precipitates the RNA and making it possible to isolate the RNA through decantation
A

-This extracts the nucleic acid and water-soluble proteins and inactivates the nucleases.

17
Q

Which of the following can be used in RNA Acid hydrolysis?

  • H2SO4
  • HCl
  • NaOH
A

-H2SO4

18
Q

True or False

If the sample is a hexose, it will give a blue-green solution in Bial’s test.

A

-False (The sample is a Pentose)

19
Q

This reagent is used in the preparation of yeast RNA to extract the nucleic acid and water-soluble proteins and inactivate nucleases

  • Ethanol
  • HCl
  • NaOH
  • Sodium Hydroxide
A

-Sodium Hydroxide

20
Q

This type of nucleic acid is double stranded and considered a carrier of genetic information

  • RNA
  • DNA
  • mRNA
  • Genes
A

-DNA

21
Q

Identify the correct pairing
Match or Mismatch

Molisch Test : test for reducing sugar

A

-Mismatch (Test for Carbohydrates)

22
Q

Identify the correct pairing
Match or Mismatch

NH4OH & AgNO3 : test for Purines

A

-Match

23
Q

Identify the correct pairing
Match or Mismatch

NH4OH, HNO3 & Ammonium molybdate : test for inorganic phosphates

A

-Match

24
Q

Identify the correct pairing
Match or Mismatch

Bial’s Orcinol reagent : test for pentose

A

-Match

25
Q

Identify the correct pairing
Match or Mismatch

Yeast RNA is insoluble in NaOH but soluble in HCl

A

-Match

26
Q

Identify the correct pairing
Match or Mismatch

Hydrolyzed RNA will give a yellow precipitate in test for phosphates and a purple ring in benedict’s test

A

-Match

27
Q

Identify the correct pairing
Match or Mismatch

Yeast RNA is insoluble in hot water but soluble in NaOH

A

-Match