M9 Flashcards
In performing the analysis of Triglycerides, the result showed a value of 215 mg/dL. What does it interpret, in terms of the NCEP classifications in the risk of coronary heart disease development?
None of these presents the correct interpretation.
The patient has low values of Triglyceride and is not at risk for coronary heart disease
The data signifies that the patient likely to develop coronary heart disease.
It means that the patient is normal and is not at risk for coronary heart disease
The data signifies that the patient likely to develop coronary heart disease.
A patient’s cholesterol level is about to be determined by a laboratory analyst using an icteric sample. The procedure states that the absorbance should be measured spectrophotometrically after incubation at room temperature for 10-15 minutes. After the addition of 1 mL total cholesterol reagent to 100 uL serum, the analyst allowed the mixture to stand at 8:35AM. After incubation, the absorbance was read at 9:15AM. Based on the performance of the procedure, why and how do you think will the results be affected?
The level of total cholesterol will be falsely elevated due to prolonged incubation.
The level of total cholesterol will be falsely decreased due to the anticoagulant.
The level of total cholesterol will be falsely suppressed due to exposure to light.
The level of total cholesterol will be falsely increased due to elevated bilirubin concentration.
The level of total cholesterol will be falsely elevated due to prolonged incubation.
What is the conversion factor for LDL cholesterol?
0.113
0.259
0.0113
0.026
-0.026
Allen, a seafarer who is a known hypertensive has to undergo medical check-up before going on board. His Total Cholesterol is 6.6 mmol/L; Triglyceride is 3.3 mmol/L and HDL is 0.80 mmol/L. What would be the value of his LDL?
4.63 mmol/L
5.29 mmol/L
5.14 mmol/L
4.28 mmol/L
-4.28 mmol/L
Saanvi, the phlebotomist on duty at 6 a.m., was assigned the task of drawing blood from a patient named Ben Stone. His lab test requests included cholesterol, creatinine, BUN, SGPT, and SOT. When he inquired about Ben’s most recent meal, she concluded that he had only fasted for about 8 hours. What is the most appropriate course of action?
Continue with the collection, noting on the requisition slip that the patient did not complete the required fasting time.
Inform the patient that he will have to wait another hour to have his blood drawn because he did not fast for the required hour.
Tell the patient that he needs to come back tomorrow to get his blood drawn because he did not fast for the required hour.
Proceed with sample collection because the tests do not require fasting.
-Proceed with sample collection because the tests do not require fasting.
Which of the following best supports the importance of purification process?
None of the choices is correct
It extracts lipids
It releases cholesterol from lipoproteins
Involves precipitation of free cholesterol remove errors
-Involves precipitation of free cholesterol remove errors
What is considered as the CDC standard method for non-enzymatic cholesterol determination?
Parek Jung
Pearson
Bloors
Abell-Kendall
-Abell-Kendall
What is the colorimetric indicator used in the CHOD-PAP method?
Quinoneimine
Antipyrine
Phenol
Peroxidase
-Quinoneimine
Which of the following differentiates Lieberman and Salkowski methods?
Lieberman uses HyS04 and acetic anhydride while Salkowski H2S04 and ferric ions
Lieberman is for colorimetric development while Salkowski is for extraction
Lieberman yields an unstable green disulfonic acid while Salkowski will result to a stable red monosulfonic acid
Lieberman is use in cholesterol determination while Salkowski is for triglycerides
-Lieberman yields an unstable green disulfonic acid while Salkowski will result to a stable red monosulfonic acid
What is added to NADH and tetrazolium dye to produce Formazan and NAD?
Glycerol Phosphate dehydrogenase
Pyruvate Kinase
Diaphorase
Lipase
-Diaphorase
A triglyceride analysis on a patient that has eaten an hour before the blood extraction would bear which results and what is the proper step to do?
Results will present a normal level of triglyceride; test results will be released.
Results will have a low value of the analyte; test should be repeated after the patient has fasted for at least 12 hours.
Results will show high levels of high levels triglycerides; test can be released as is.
Results will show very high levels of triglyceride; test should be repeated after proper fasting is followed.
-Results will show very high levels of triglyceride; test should be repeated after proper fasting is followed.
Which of these can be used to extract triglycerides from plasma?
Potassium Hydroxide
Heparin sulfate
Magnesium aluminum silicate
Trichloroacetic acid
Magnesium aluminum silicate
Choose the correct statement that will best describe the saponification step during triglyceride determination.
It will aid in the measurement of compounds that forms.
A pretreatment of sample to remove the lipids from proteins.
Carried out in order to convert glycerol into a measurable compound.
It is done to cleave the TAG molecule into fatty acids and glycerol.
It is done to cleave the TAG molecule into fatty acids and glycerol.
Which of the following statements are true about HDLc measurement?
1. Chylomicrons, very low density and high-density lipoproteins in the sample are precipitated by phosphotungstic acid and magnesium ions
2. After centrifugation, high density lipoproteins are in the precipitate.
3. Chylomicrons, very low density and low-density lipoproteins in the sample are precipitated by phosphotungstic acid and magnesium ions.
4. Cholesterol in the supernatant is measured by an enzymatic method.
All of the statements are correct
Ill & IV
I, II & Ill
II & IV
-Ill & IV
Which formula will be used to calculate the VLDL value in the SI unit?
Triglyceride value divided by 0.0113
Triglyceride value divided by 2.2
Triglyceride value divided by 5
Triglyceride value multiplied by 0.0113
Triglyceride value divided by 2.2
