M8 Flashcards
Loise performs radial immunodiffusion by batch on several patients for Complement 3. After 72 hours of incubation, the precipitin rings formed are shown in the image below. Which patient would have the highest concentration of the said protein?
Patient #13
Patient #12
Patient #11
Patient #14
-Patient #13
Which of the following are the expected visible results when tryptophan present in globulin is allowed to react directly with glyoxylic acid and sulfuric acid?
Orange
Red
Purple
Green
-Purple
What is being measured in the Kjeldahl method?
None of the choices is correct.
Measures the refractive index
Amount of nitrogen in the specimen
Formation of cu2+ ions and peptide bonds
Amount of nitrogen in the specimen
A 55-year-old patient submitted for blood extraction for his annual check-up. The results below are expressed in the conventional unit, determine the globulin concentration in Sl unit.
Total Protein - 7.51 Albumin - 5.55
19.6 mg%
0.196 g/dL
19.6 g/L
1.96 g/dL
19.6 g/L
What is the end product of the colorimetric method for globulin determination?
Pink
Green
Orange
Purple
Purple
In radial immunodiffusion, how is protein quantitated?
By measuring the Ag-Ab precipitate length
By measuring reflected light
By comparing the size, shape, density, and location of the arcs of the patient sample with the arcs of the quality control sample
The diameter of the precipitin ring is plotted against the antigen concentration on a logarithmic scale
The diameter of the precipitin ring is plotted against the antigen concentration on a logarithmic scale
During the Kjeldahl process, the medical technologist used CA to precipitate the proteins. The protein pellet undergoes a digestion step using H2S04 with heat and then a copper sulfate is added. What is the function of copper sulfate?
Serves as catalyst and accelerate the reaction
Improves efficiency of digestion step
Increase the boiling point
All of the choices are correct
Serves as catalyst and accelerate the reaction
Cal visited the laboratory for his annual check-up. What is Cal’s globulin level in g/dL based on his laboratory results?
32 g/dL
82 g/dL
8.2 g/dL
3.2 g/dL
-3.2 g/dL
What is being measured in the salt fractionation method?
Albumin
Globulin
Total Protein
Both A and B
-Albumin
Based on the electrophoretic pattern, what is the possible diagnosis of the patient?
Acute inflammation
Multiple Myeloma
Liver Cirrhosis
Nephrotic Syndrome
-Multiple Myeloma
Which of the following raises the boiling point of H2SO4 while CuSO4 catalyzes the reaction?
K2SO4
Na2SO4
BaSO4
All of the choices are correct.
-K2SO4
Below is a blood sample taken from a 55-year-old patient. If the sample will be used for laboratory analysis, from which of the following methods will albumin level be falsely suppressed?
Refractometry
Kjeldahl Method
Salt Fractionation
HABA Dye-binding method
-HABA Dye-binding method
Which of the following protein functions below is incorrect?
Aiding digestion and increasing bioavailability
Catalyzing biochemical reactions
Transporting metals such as iron and copper
Acting as receptors for hormones
-Aiding digestion and increasing bioavailability
What are the characteristics of the bands found in protein electrophoresis by analyzing a serum of a patient with multiple myeloma?
All bands are stained palely.
The bands at the center are thick and dark while the two ends are narrow and light in staining.
The band nearest to the anode is the thickest while the following bands are narrow and are pale.
Bands in both ends are dark and thick, while the other bands narrow and lightly stained.
-Bands in both ends are dark and thick, while the other bands narrow and lightly stained.
Which of the following will have the narrowest band in protein electrophoresis?
Alpha 1 globulins
Gamma globulins
Beta globulins
Alpha 2 globulins
Alpha 1 globulins
