M6 Flashcards
butterfly-shaped gland consisting of 2 lobes, located below the larynx
THYROID GLAND
Produce metabolic hormones (D3 and D4)
Follicular Cells
produce calcitonin for the regulation of calcium and phosphate
Parafollicular cells or C cells:
Sandwich method
Detects the antigen
Uses 2 different antibodies for the same target antigen
Antibodies will bind to different epitopes of the antigen
Measures through spectrophotometrically
Most sensitive method
RIA (RADIO IMMUNOASSAY)
Competitive binding
RIA (RADIO IMMUNOASSAY)
An antigen competes with a non-radioactive antigen for a fixed number of antibody receptor or binding sites.
RIA (RADIO IMMUNOASSAY)
In the laboratory, machine used is ___ for the detection of TSH
miniVIDAS
Principle of miniVIDAS:
follows the same principle of ELFA or Enzyme Linked Fluorescent Assay
Used to confirm borderline cases of Graves or Hashimoto’s diseases, or diseases for thyroidism.
TRH STIMULATION TEST
Differentiates secondary (pituitary) from tertiary (hypothalamic) hypothyroidism
TRH STIMULATION TEST
stable for 5 days at 2 to 8℃, and or at least 1 month when stored frozen
TSH
For newborn screening, whole blood may be collected by heel puncture ____after birth
48 to 72 hours
most metabolically active thyroid hormonal activity
T3
